好斗的
生物
应力颗粒
细胞生物学
泛素
MG132型
HEK 293细胞
蛋白酶体
遗传学
蛋白酶体抑制剂
细胞培养
信使核糖核酸
基因
翻译(生物学)
作者
John Ho Chun Lai,Marianthi Tsogka,Jun Xia
标识
DOI:10.1091/mbc.e24-05-0201
摘要
The aggresome is a perinuclear structure that sequesters misfolded proteins. It's implicated in various neurodegenerative diseases. The perinuclear structure enriched with PICK1 was found to be inducible by cellular stressors, colocalizing with microtubule-organizing center (MTOC) markers and ubiquitin, hence classifying it as an aggresome. Sodium arsenite but not arsenate was found to potently induce aggresome formation through an integrated stress response (ISR)-independent pathway. In HEK293T cells, under arsenite stress, PICK1 localization to the aggresome was prioritized, and formation of PICK1 homodimers was favored. Additionally, PICK1 could enhance protein entry into aggresomes. This study shows that arsenite can induce the formation of both RNA stress granules and aggresomes at the same time, and that PICK1 shows conditional localization to aggresomes, suggesting a possible involvement of PICK1 in neurodegenerative diseases. [Media: see text] [Media: see text]
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