The prognostic and immune significance of Rab11A in pan‐cancer and its function and mechanism underlying estrogen receptor targeting in breast cancer

生物 癌症研究 乳腺癌 基因敲除 转移 雌激素受体 癌症 内科学 肿瘤科 医学 细胞培养 遗传学
作者
Yilun Li,Baifang Ding,Mengyu Wei,Xiaolu Yang,Ruihuan Fu,Yinfeng Liu,Lin Zhu,Yan Ding,Wenjin Zhang,Geng Zhang,Shuo Zhang,Yuhui Bu,Jianchao He,Jianye Deng,Xiaohuan Bao,Jun Hao,Li Ma
出处
期刊:Asia-pacific Journal of Clinical Oncology [Wiley]
标识
DOI:10.1111/ajco.14130
摘要

Abstract Objective Rab11A is an important molecule for recycling endosomes and is closely related to the proliferation, invasion, and metastasis of tumors. This study investigated the prognostic and immune significance of Rab11A and validated its potential function and mechanism in breast cancer (BRCA). Methods RNA sequencing data for 33 tumors were downloaded from The Cancer Genome Atlas (TCGA) and Genotype‐Tissue Expression databases. Correlation analysis was used to evaluate the relationship between Rab11A expression and immune characteristics. Potential pathways were identified using the Kyoto Encyclopedia of Genes and Genomes and Gene Ontology analysis. Immunohistochemical analysis, colony formation assay, bromodeoxyuridine incorporation assay, immunofluorescence, and Western blot were used to explore potential function and mechanism. Results Analysis of the TCGA database showed significant upregulation of Rab11A expression in a variety of cancers. Rab11A was up‐regulated in 82.4% of BRCA. High Rab11A expression is associated with poor survival in cancer patients and is a predictor of poor prognosis. CIBERSORT analysis showed that Rab11A was negatively associated with almost all immune cycle activity scores pan‐cancer. The results of the TCGA‐BRCA cohort were further confirmed by using pathological samples from clinical BRCA patients. The results showed that Rab11A expression was correlated with estrogen receptor (ER) and progesterone receptor expression in BRCA ( p < 0.05). Knockdown and overexpression of Rab11A affected the proliferation of BRCA cells. Further mechanistic studies revealed that down‐regulation of ER alpha (ERα) and up‐regulation of ER beta (ERβ) mediated Rab11A‐induced inhibition of BRCA cell proliferation. Conclusion Rab11A expression in pan‐cancer is associated with poor prognosis and immune profile. In particular, in BRCA, Rab11A expression regulates cell proliferation by targeting ERα and ERβ. High Rab11A expression is tightly associated with immune characteristics, tumor microenvironment, and genetic mutations. These results provide a reference for exploring the role of Rab11A in pan‐cancer and provide a new perspective for revealing potential therapeutic targets in BRCA.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
我是老大应助西瓜汁采纳,获得30
刚刚
王星星发布了新的文献求助10
1秒前
pluto应助zhangyaoyang采纳,获得10
2秒前
阿鑫完成签到 ,获得积分10
2秒前
语梦完成签到,获得积分10
2秒前
znn完成签到 ,获得积分10
2秒前
精明怜南发布了新的文献求助10
3秒前
szc-2000发布了新的文献求助10
3秒前
斯文败类应助走走道疯了采纳,获得10
4秒前
4秒前
无花果应助王星星采纳,获得10
8秒前
superfatcat完成签到,获得积分10
8秒前
苏雅霏完成签到 ,获得积分10
9秒前
EED发布了新的文献求助10
10秒前
11秒前
11秒前
cindywu发布了新的文献求助10
13秒前
科研通AI2S应助Huang_being采纳,获得10
13秒前
13秒前
14秒前
量子星尘发布了新的文献求助10
14秒前
HCB1发布了新的文献求助10
15秒前
15秒前
ShuXU完成签到,获得积分10
17秒前
17秒前
Pom完成签到,获得积分10
17秒前
gujiguji发布了新的文献求助10
19秒前
科研通AI2S应助HCB1采纳,获得10
19秒前
HOME发布了新的文献求助30
20秒前
阿俊发布了新的文献求助10
20秒前
22秒前
Richard发布了新的文献求助10
22秒前
FashionBoy应助sanyecai采纳,获得10
25秒前
25秒前
26秒前
科目三应助gujiguji采纳,获得10
26秒前
advance发布了新的文献求助10
28秒前
29秒前
29秒前
小二郎应助科研通管家采纳,获得10
29秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3988920
求助须知:如何正确求助?哪些是违规求助? 3531290
关于积分的说明 11253247
捐赠科研通 3269903
什么是DOI,文献DOI怎么找? 1804830
邀请新用户注册赠送积分活动 882027
科研通“疑难数据库(出版商)”最低求助积分说明 809052