乙胺
茶氨酸
活性氧
化学
渗透性休克
生物化学
脱落酸
山茶
转录因子
细胞生物学
生物
植物
食品科学
绿茶
基因
有机化学
作者
Ziwen Zhou,Xiaoguang Luo,Maoyin Fu,Siya Li,Yaohua Cheng,Yeyun Li,Xianchen Zhang
摘要
SUMMARY The tea plant ( Camellia sinensis ) is a perennial green plant, and its tender leaves are rich in secondary metabolites, such as theanine. Ethylamine (EA), a small amine, is an important prerequisite for theanine synthesis. However, beyond its involvement in theanine synthesis, the other physiological functions of EA in tea plants remain unknown. In vitro experiments indicate that EA may function as scavengers of reactive oxygen species (ROS) to protect the plant against damage caused by osmotic stress. Additionally, a significant correlation between EA levels and osmotic tolerance has been observed in different tea varieties. From the results, alanine decarboxylase ( CsAlaDC )‐silenced tea leaves and overexpressed CsAlaDC Arabidopsis thaliana lines decreased and increased EA levels, respectively, and mediated ROS homeostasis, thus exhibiting a sensitive and tolerant phenotype. In addition, the transcription factor (TF) CsCBF4 was functionally identified, which can directly bind to the CsAlaDC promoter. CsCBF4‐ silenced tea leaves significantly reduced the expression levels of CsAlaDC and in turn EA content , resulting in excess ROS accumulation and an osmotic‐sensitive phenotype. Taken together, these results established a new regulatory module consisting of CBF4‐ CsAlaDC responsible for EA accumulation and ROS homeostasis in response to osmotic stress.
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