CRISPR/Cas-based Aptasensor as an Innovative Sensing Approaches for Food Safety Analysis: Recent Progresses and New Horizons

清脆的 适体 生化工程 食品安全 风险分析(工程) 计算机科学 纳米技术 计算生物学 生物技术 业务 工程类 化学 生物 材料科学 生物化学 遗传学 食品科学 基因
作者
Marwah Suliman Maashi
出处
期刊:Critical Reviews in Analytical Chemistry [Taylor & Francis]
卷期号:54 (7): 2599-2617 被引量:16
标识
DOI:10.1080/10408347.2023.2188955
摘要

Food safety is one of the greatest public problems occurring around the world. Chemical, physical, and microbiological hazards could lead to food safety problems, which might occur at all stages of the supply chain. To tackle food safety problems and protect consumer health, specific, accurate, and rapid diagnosis techniques meeting various requirements are the imperative measures to ensure food safety. CRISPR-Cas system, a novel emerging technology, is effectively repurposed in (bio)sensing and has shown a tremendous capability to develop on-site and portable diagnostic methods with high specificity and sensitivity. Among numerous existing CRISPR/Cas systems, CRISPR/Cas13a and CRISPR/Cas12a are extensively employed in the design of biosensors, owing to their ability to cleave both non-target and target sequences. However, the specificity limitation in CRISPR/Cas has hindered its progress. Nowadays, nucleic acid aptamers recognized for their specificity and high-affinity characteristics for their analytes are incorporated into CRISPR/Cas systems. With the benefits of reproducibility, high durability, portability, facile operation, and cost-effectiveness, CRISPR/Cas-based aptasensing approaches are an ideal choice for fabricating highly specific point-of-need analytical tools with enhanced response signals. In the current study, we explore some of the most recent progress in the CRISPR/Cas-mediated aptasensors for detecting food risk factors including veterinary drugs, pesticide residues, pathogens, mycotoxins, heavy metals, illegal additives, food additives, and other contaminants. The nanomaterial engineering support with CRISPR/Cas aptasensors is also signified to achieve a hopeful perspective to provide new straightforward test kits toward trace amounts of different contaminants encountered in food samples.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
量子星尘发布了新的文献求助10
刚刚
affy210310完成签到,获得积分10
1秒前
名字不好起完成签到,获得积分10
1秒前
齐朕完成签到,获得积分10
2秒前
大橙子发布了新的文献求助10
4秒前
speed完成签到 ,获得积分10
5秒前
RYK完成签到 ,获得积分10
6秒前
7秒前
cm发布了新的文献求助10
11秒前
13秒前
13秒前
13秒前
13秒前
13秒前
大力水手完成签到,获得积分0
15秒前
17秒前
cm完成签到,获得积分10
19秒前
酷炫的听枫完成签到 ,获得积分10
20秒前
吱吱吱完成签到 ,获得积分10
21秒前
22秒前
上善若水呦完成签到 ,获得积分10
22秒前
小羡完成签到 ,获得积分10
22秒前
cqwswfl完成签到 ,获得积分20
23秒前
南山无梅落完成签到,获得积分10
24秒前
啵妞完成签到 ,获得积分10
24秒前
上官若男应助qiqi采纳,获得30
26秒前
拼搏的潘子完成签到,获得积分10
27秒前
zsj完成签到,获得积分10
28秒前
dolesy发布了新的文献求助10
29秒前
执着烧鹅完成签到 ,获得积分10
29秒前
哈哈哈完成签到,获得积分10
31秒前
yar应助博修采纳,获得10
33秒前
可爱的函函应助博修采纳,获得10
33秒前
MchemG应助博修采纳,获得10
33秒前
酷波er应助博修采纳,获得10
34秒前
时代更迭完成签到 ,获得积分10
34秒前
35秒前
WGOIST完成签到,获得积分10
36秒前
九九完成签到 ,获得积分10
36秒前
李新宇完成签到 ,获得积分10
37秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038157
求助须知:如何正确求助?哪些是违规求助? 3575869
关于积分的说明 11373842
捐赠科研通 3305650
什么是DOI,文献DOI怎么找? 1819255
邀请新用户注册赠送积分活动 892655
科研通“疑难数据库(出版商)”最低求助积分说明 815022