Enhancement of the Peroxidase Activity of g-C3N4 with Different Morphologies for Simultaneous Detection of Multiple Antibiotics

The classes and forms of antibiotics directly determine their ecotoxicity and environmental chemical behavior, and developing a sensor array for simultaneous and in situ detection of antibiotics is highly anticipated. In this study, different morphologies of g-C3N4 with different fluorescence properties and peroxidase activity were prepared by regulating the degree of interlayer stacking and planar connectivity. Subsequently, in order to enhance its enzyme activity and amplify the differences in response signals to different antibiotics, three morphologies of g-C3N4/MIL-101(Fe) were prepared by in situ growth of equivalent amounts of MIL-101(Fe) on g-C3N4, respectively. The sensor array constructed based on the cross-response signals between g-C3N4/MIL-101(Fe) and antibiotics not only realized the simultaneous detection of quinolones, furans, tetracyclines, and lincomamides but also could efficiently identify their seven different forms. In the range of 0.2-0.8 ppm, the minimum detection limit for antibiotics was 12 ppb. In addition, the recovery experiments of multicomponent-mixed antibiotics in environmental samples show that the recovery rate remained at 91.42-107.59%, confirming the reliability and practicality of the sensor array. This study not only revealed the influence of crystal morphology regulation on the optical properties and enzyme activities of nanozymes, but also provided support for tracing, ecological remediation, and in situ environmental chemical behavior research of antibiotics.