作者
Jing Cao,Wenjuan Li,Jie Li,Md. Abdul Mazid,Chunyang Li,Yu Jiang,Wenqi Jia,Liang Wu,Zhaodi Liao,Shiyu Sun,Weixiang Song,Jiqiang Fu,Yan Wang,Yong Lu,Yuting Xu,Yanhong Nie,Xinyan Bian,Changshan Gao,Xiaotong Zhang,Liansheng Zhang,Shenshen Shang,Yunpan Li,Lixin Fu,Hao Liu,Junjian Lai,Yang Wang,Yue Yuan,Xin Jin,Yan Li,Chuanyu Liu,Yiwei Lai,Xuyang Shi,Patrick H. Maxwell,Xun Xu,Longqi Liu,Mu‐ming Poo,Xiaolong Wang,Qiang Sun,Miguel A. Esteban,Zhen Liu
摘要
A formal demonstration that mammalian pluripotent stem cells possess preimplantation embryonic cell-like (naive) pluripotency is the generation of chimeric animals through early embryo complementation with homologous cells. Whereas such naive pluripotency has been well demonstrated in rodents, poor chimerism has been achieved in other species including non-human primates due to the inability of the donor cells to match the developmental state of the host embryos. Here, we have systematically tested various culture conditions for establishing monkey naive embryonic stem cells and optimized the procedures for chimeric embryo culture. This approach generated an aborted fetus and a live chimeric monkey with high donor cell contribution. A stringent characterization pipeline demonstrated that donor cells efficiently (up to 90%) incorporated into various tissues (including the gonads and placenta) of the chimeric monkeys. Our results have major implications for the study of primate naive pluripotency and genetic engineering of non-human primates.