Intelligent quantitative recognition of sulfide using machine learning-based ratiometric fluorescence probe of metal-organic framework UiO-66-NH2/Ppix

Sulfides possess either high toxicity or play crucial physiological role such as gas transmitter dependent upon dosage, hence the significant for their rapid sensitive and selective concentration determination. Herein, a machine learning enhanced ratiometric fluorescence sensor was engineered for sulfide determination by incorporating the nanometal-organic framework (UiO-66-NH2) along with protoporphyrin IX (Ppix). The blue fluorescence at 431 nm originated from the moiety of UiO-66-NH2 by 365 nm excitation serves as an internal calibration reference signal, while the red fluorescence at 629 nm from the moiety of Ppix serves as the analytical signal, and the intensity is correlated to the amount of sulfides. The fluorescence color of the sensor gradually varies from blue to red upon sequential addition of copper and sulfide ions, resulting in RGB (Red, Green, Blue) feature values for corresponding sulfide concentrations, which facilities the advanced data processing techniques using machine learning algorithms. On the basis of fluorescence image fingerprint extraction and machine learning algorithms, an online data analysis model was developed to improve the precision and accuracy of sulfide determination. The established model employed Linear Discriminant Analysis (LDA) and was subjected to rigorous cross-validation to ensure its robustness. By analyzing the correlation between RGB feature values and sulfide concentrations, the study highlighted a significant positive relationship between the red feature values and sulfide concentrations. The application of machine learning techniques on the ratiometric fluorescence signal of the UiO-66-NH2/Ppix probe demonstrated its potential for intelligent quantitative determination of sulfides, offering a valuable and efficient tool for pollution detection and real-time rapid environmental monitoring.