神经科学
血清素
导水管周围灰质
扁桃形结构
光遗传学
加巴能
抑制性突触后电位
5-羟色胺受体
5-羟色胺能
杏仁核
生物
心理学
医学
受体
内科学
中枢神经系统
中脑
作者
Olivia J. Hon,Jeffrey F. DiBerto,Christopher M. Mazzone,Jonathan A. Sugam,Daniel W. Bloodgood,J. Andrew Hardaway,Mariya Husain,Alexis Kendra,Nora M. McCall,Alberto J. López,Thomas L. Kash,Emily G. Lowery-Gionta
标识
DOI:10.1038/s41386-022-01392-4
摘要
Fear is an adaptive state that drives defensive behavioral responses to specific and imminent threats. The central nucleus of the amygdala (CeA) is a critical site of adaptations that are required for the acquisition and expression of fear, in part due to alterations in the activity of inputs to the CeA. Here, we characterize a novel GABAergic input to the CeA from the ventral periaqueductal gray (vPAG) using fiber photometry and ex vivo whole-cell slice electrophysiology combined with optogenetics and pharmacology. GABA transmission from this ascending vPAG-CeA input was enhanced by serotonin via activation of serotonin type 2 C (5HT2C) receptors. Results suggest that these receptors are presynaptic. Interestingly, we found that GABA release from the vPAG-CeA input is enhanced following fear learning via activation of 5HT2C receptors and that this pathway is dynamically engaged in response to aversive stimuli. Additionally, we characterized serotonin release in the CeA during fear learning and recall for the first time using fiber photometry coupled to a serotonin biosensor. Together, these findings describe a mechanism by which serotonin modulates GABA release from ascending vPAG GABA inputs to the CeA and characterize a role for this pathway in fear.
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