沙门氏菌
清脆的
化学
细菌
聚合酶链反应
微生物学
质粒
DNA连接酶
连接酶连锁反应
分子生物学
生物
DNA
多重聚合酶链反应
基因
遗传学
生物化学
作者
Changyu Zhou,Wenjing Li,Yu Zhao,Kui Gu,Ziwei Liao,Boyan Guo,Zheren Huang,Ming Yang,Hongcheng Wei,Peng Ma,Chao Li,Hao Li,Yizhi Tang,Changwei Lei,Hongning Wang
标识
DOI:10.1016/j.aca.2023.340885
摘要
Several viable Salmonella bacteria are capable of causing severe human diseases and huge economic losses. In this regard, viable Salmonella bacteria detection techniques that can identify small numbers of microbial cells are highly valuable. Here, we present a detection method (referred to as SPC) based on the amplification of tertiary signals using splintR ligase ligation, PCR amplification and CRISPR/Cas12a cleavage. The detection limit of the SPC assay was 6 copies (HilA RNA) and 10 CFU (cell). Based on Intracellular HilA RNA detection, this assay can be used to distinguish between viable and dead Salmonella. In addition, it is able to detect multiple serotypes of Salmonella and has been successfully used to detect Salmonella in milk or isolated from farms. Overall, this assay is a promising test for viable pathogens detection and biosafety control.
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