噬菌体
毒力
铜绿假单胞菌
噬菌体疗法
微生物学
噬菌体
生物
生物膜
基因组
基因
细菌
遗传学
大肠杆菌
作者
Shuai Le,Leilei Wei,Jing Wang,Tian Fang,Qian Yang,Jingru Zhao,Zhuojun Zhong,Jiazhen Liu,Xuesong He,Qiu Zhong,Shuguang Lu,Haihua Liang
出处
期刊:Nature microbiology
日期:2024-06-17
卷期号:9 (7): 1828-1841
标识
DOI:10.1038/s41564-024-01719-5
摘要
Bacteriophages have evolved diverse strategies to overcome host defence mechanisms and to redirect host metabolism to ensure successful propagation. Here we identify a phage protein named Dap1 from Pseudomonas aeruginosa phage PaoP5 that both modulates bacterial host behaviour and contributes to phage fitness. We show that expression of Dap1 in P. aeruginosa reduces bacterial motility and promotes biofilm formation through interference with DipA, a c-di-GMP phosphodiesterase, which causes an increase in c-di-GMP levels that trigger phenotypic changes. Results also show that deletion of dap1 in PaoP5 significantly reduces genome packaging. In this case, Dap1 directly binds to phage HNH endonuclease, prohibiting host Lon-mediated HNH degradation and promoting phage genome packaging. Moreover, PaoP5Δdap1 fails to rescue P. aeruginosa-infected mice, implying the significance of dap1 in phage therapy. Overall, these results highlight remarkable dual functionality in a phage protein, enabling the modulation of host behaviours and ensuring phage fitness.
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