Diverse intratumoral heterogeneity and immune microenvironment of two HPV‐related cervical cancer types revealed by single‐cell RNA sequencing

肿瘤微环境 生物 癌症研究 CD8型 细胞毒性T细胞 免疫疗法 免疫系统 细胞 免疫学 遗传学 生物化学 体外
作者
Chunbo Li,Danyang Liu,Yu Zhao,Yan Ding,Keqin Hua
出处
期刊:Journal of Medical Virology [Wiley]
卷期号:95 (6) 被引量:7
标识
DOI:10.1002/jmv.28857
摘要

Cervical squamous cell carcinoma (SCC) and adenocarcinoma (AD) are the main histological types of human papillomavirus-related cervical cancer. However, there are few reports on cell type-specific molecular differences between SCC and AD. Here, we used unbiased droplet-based single-cell RNA sequencing to elucidate the cellular differences between SCC and AD in tumor heterogeneity, and tumor microenvironment (TME). A total of 61 723 cells from three SCC and three AD patients, were collected and divided into nine cell types. Epithelial cells exhibited high intra- and interpatient heterogeneity and functional diversity. Signaling pathways, such as epithelial-to-mesenchymal-transition (EMT), hypoxia and inflammatory response were upregulated in SCC, while cell cycle-related signaling pathways were highly enriched in AD. SCC was associated with high infiltration of cytotoxicity CD8 T, effector memory CD8 T, proliferative natural killer (NK), and CD160+ NK cells as well as tumor-associated macrophages (TAMs) with high major histocompatibility complex-II genes. AD exhibited a high proportion of naive CD8 T, naive CD4 T, Treg CD4, central memory CD8, and TAMs with immunomodulatory functions. Additionally, we also observed that the majority of cancer-associated fibroblasts (CAFs) were from AD, and participated in inflammation regulation, while SCC-derived CAFs exhibited similar functions to tumor cells, such as EMT and hypoxia. This study revealed the widespread reprogramming of multiple cell populations in SCC and AD, dissected the cellular heterogeneity and characteristics in TME, and proposed potential therapeutic strategies for CC, such as targeted therapy and immunotherapy.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
GG完成签到,获得积分10
刚刚
科研通AI2S应助Llllllxxxxxxx采纳,获得10
刚刚
刚刚
上官若男应助小侯采纳,获得10
刚刚
半醉哥完成签到,获得积分10
刚刚
1秒前
烟花应助脱壳金蝉采纳,获得10
1秒前
scc完成签到,获得积分10
1秒前
VAIN39完成签到,获得积分10
1秒前
寒暄half完成签到 ,获得积分10
2秒前
jasmine发布了新的文献求助10
3秒前
一禅完成签到 ,获得积分10
3秒前
4秒前
5秒前
pureivy22完成签到 ,获得积分10
5秒前
忧伤的元菱完成签到 ,获得积分20
5秒前
shit完成签到 ,获得积分10
6秒前
啦啦啦发布了新的文献求助10
7秒前
7秒前
整齐乐巧发布了新的文献求助30
7秒前
suger发布了新的文献求助10
8秒前
善学以致用应助黄黄黄采纳,获得10
8秒前
8秒前
斯文的小鸭子关注了科研通微信公众号
9秒前
YahuiZhou完成签到,获得积分10
9秒前
任ren发布了新的文献求助10
9秒前
墨西哥范闲完成签到,获得积分10
9秒前
搞科研的静静完成签到,获得积分10
9秒前
9秒前
好困应助Regina采纳,获得20
10秒前
10秒前
帅冰冰冰完成签到,获得积分10
10秒前
大山完成签到 ,获得积分10
10秒前
11秒前
lllllll完成签到,获得积分10
11秒前
大艺术家完成签到,获得积分10
11秒前
结草衔环完成签到,获得积分10
11秒前
12秒前
12秒前
星辰坠于海完成签到,获得积分0
12秒前
高分求助中
Sustainability in Tides Chemistry 2000
Microlepidoptera Palaearctica, Volumes 1 and 3 - 13 (12-Volume Set) [German] 1122
Дружба 友好报 (1957-1958) 1000
The Data Economy: Tools and Applications 1000
Mantiden - Faszinierende Lauerjäger – Buch gebraucht kaufen 700
PraxisRatgeber Mantiden., faszinierende Lauerjäger. – Buch gebraucht kaufe 700
A Dissection Guide & Atlas to the Rabbit 600
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3099988
求助须知:如何正确求助?哪些是违规求助? 2751451
关于积分的说明 7614528
捐赠科研通 2403644
什么是DOI,文献DOI怎么找? 1275290
科研通“疑难数据库(出版商)”最低求助积分说明 616351
版权声明 599053