Antiviral Activity and Underlying Mechanisms of Baicalin Against Porcine Reproductive and Respiratory Syndrome Virus in Vitro

Download This Paper Open PDF in Browser Add Paper to My Library Share: Permalink Using these links will ensure access to this page indefinitely Copy URL Antiviral Activity and Underlying Mechanisms of Baicalin Against Porcine Reproductive and Respiratory Syndrome Virus in Vitro 26 Pages Posted: 27 Feb 2024 See all articles by Weichen ChangWeichen ChangHenan Agricultural UniversityJing WuHenan Agricultural UniversityFeifan WuHenan Agricultural UniversityHong-Ying ZhangHenan Agricultural UniversityMingfan YangHenan Agricultural University Abstract Porcine reproductive and respiratory syndrome (PRRS) is a major challenge for the global swine industry, causing huge economic losses worldwide. To date, there are no effective measures to prevent and control the spread of PRRS virus (PRRSV). Baicalin (BA) is a natural flavonoid with various pharmacological effects, including antiviral, anti-inflammatory, antioxidant and immunomodulatory. Here, we demonstrate that BA exhibits potent anti-PRRSV activity in vitro, BA concentrations in the range of 5-20 μg/mL significantly inhibited PRRSV infection in a dose-dependent manner and were independent of PRRSV strain. Mechanistically, BA inhibited PRRSV replication by directly interacting with virions, thereby affecting multiple stages of the virus life cycle. Meanwhile, the preventive effect of BA on PRRSV could be realized by inhibiting CD151 and CD163 expression. Furthermore, BA reduced the PRRSV-induced production of PAMs cytokines (IFN-α, IL-6, IL-8, and TNF-α), suggesting that BA-induced antiviral cytokines may help BA inhibit PRRSV infection. Taken together, BA can be used as an inhibitor of PRRSV infection in vitro, which provides a theoretical basis for the clinical application of BA and the prevention and control of PRRSV infection, which is worthy of further study in vivo. Note: Funding declaration: The present work was supported by the key R&D Project of Henan Province' Research on key technology of traditional Chinese medicine and its application in healthy breeding of livestock and poultry ' (No. 221111111300). Conflict of Interests: The authors declare no conflict of interest. Keywords: Baicalin, porcine reproductive and respiratory syndrome virus, Antiviral effect, Cytokine Suggested Citation: Suggested Citation Chang, Weichen and Wu, Jing and Wu, Feifan and Zhang, Hong-Ying and Yang, Mingfan, Antiviral Activity and Underlying Mechanisms of Baicalin Against Porcine Reproductive and Respiratory Syndrome Virus in Vitro. Available at SSRN: https://ssrn.com/abstract=4731615 Weichen Chang Henan Agricultural University ( email ) ZhengzhouChina Jing Wu Henan Agricultural University ( email ) ZhengzhouChina Feifan Wu Henan Agricultural University ( email ) ZhengzhouChina Hong-Ying Zhang Henan Agricultural University ( email ) ZhengzhouChina Mingfan Yang (Contact Author) Henan Agricultural University ( email ) ZhengzhouChina Download This Paper Open PDF in Browser Do you have negative results from your research you’d like to share? Submit Negative Results Paper statistics Downloads 2 Abstract Views 17 31 References PlumX Metrics Related eJournals Respiratory Medicine eJournal Follow Respiratory Medicine eJournal Subscribe to this fee journal for more curated articles on this topic FOLLOWERS 43 PAPERS 1,042 Feedback Feedback to SSRN Feedback (required) Email (required) Submit If you need immediate assistance, call 877-SSRNHelp (877 777 6435) in the United States, or +1 212 448 2500 outside of the United States, 8:30AM to 6:00PM U.S. Eastern, Monday - Friday.