脂质体
膜流动性
磷脂
膜
脂质双层
荧光各向异性
双层
小泡
化学
生物物理学
荧光
生物化学
生物
光学
物理
出处
期刊:Methods in molecular biology
日期:2023-01-01
卷期号:: 241-244
被引量:4
标识
DOI:10.1007/978-1-0716-2954-3_21
摘要
A liposome is a sealing vesicle composed of a biofilm-like phospholipid bilayer. Continuous phospholipid exchange across the membrane causes spontaneous accumulation and deposition of liposome ions, resulting in the instability of the phospholipid membrane. Effective and reliable techniques to evaluate the stability of liposomes are necessary. Specifically, 1,6-diphenyl-1,3,5-hexatriene (DPH) is a fluorescent probe commonly utilized to assess the fluidity of membranes. Membrane fluidity is inversely proportional to the fluorescence characteristic of DPH; the higher the anisotropy of DPH is, the lower is the membrane fluidity. Here, we described the DPH method for liposome-membrane fluidity determination.
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