光谱学
吸收(声学)
吸收光谱法
激发态
粘度
信号(编程语言)
化学
分析化学(期刊)
荧光
荧光光谱法
超快激光光谱学
时间分辨光谱学
灵敏度(控制系统)
材料科学
光学
原子物理学
物理
色谱法
量子力学
计算机科学
复合材料
程序设计语言
电子工程
工程类
作者
Yonggang Yang,Yang Liu,Beidou Feng,Chaochao Qin,Tiantian Guan,Ge Wang,Hua Zhang,Yufang Liu
摘要
Transient absorption (TA) signals have the features of real-time multi-signals and high sensitivity for biological monitoring, and they are less disturbed by the biological environment compared to single fluorescence signals. Two intelligently regulated probes (1-VBM and 1-VBH) are designed and synthesized with time-resolved excited-state absorption (ESA) and stimulated emission (SE) signals in TA spectroscopy. In mixed solutions of glycerol and DMSO, the ESA lifetimes of the 1-VBM probe linearly increase (y = 4.5419x + 303.98) with an increase in solvent viscosity (2.79–190.35 cP), showing similar sensitivity as SE lifetimes (y = 4.6342x + 311.06). Therefore, these TA signals can be used to monitor the viscosity of live cells. As serum changes in viscosity, 1-VBM has a sensitive and specific time-resolved ESA signal. Interestingly, the ESA signal is several times stronger than the SE signal in the serum of both healthy mice and mice with hepatoma. The lifetime of ESA signal in hepatoma serum (958.6 ps) is longer than that in normal serum (632.8 ps), which is induced by the higher blood viscosity in hepatoma mice. This indicates that the ESA signals in TA spectroscopy are a reliable marker for detecting viscosity in biological samples, and can be a potential tool for clinical diagnosis.
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