Comprehensive analysis of microbiome, metabolome and transcriptome revealed the mechanisms of Moringa oleifera polysaccharide on preventing ulcerative colitis

This study aimed to investigate the protective effect of Moringa oleifera polysaccharide (MOP) on ulcerative colitis (UC) and explore its mechanism through the combined analysis of microbiome, metabolome and transcriptome. A UC model in mice was established using dextran sulphate sodium. After a 21-day experiment, results showed that MOP could inhibit the weight loss and disease activity index in UC mice. The intervention of MOP decreased the expression of inflammatory cytokines and promoted the secretion of tight junctions. MOP could promote the growth of probiotics such as Lachnospiraceae_NK4A136, Intestinimonas and Bifidobacterium in UC mice. The results of metabolomic and transcriptomic analysis indicated that MOP could regulated the metabolism of polyunsaturated fatty acid and PPAR, TLR and TNF signalling pathways might play important roles in the process. Altogether, MOP could be used as a functional food to prevent UC.