Baicalin improved hepatic injury of NASH by regulating NRF2/HO-1/NRLP3 pathway

黄芩苷 脂肪肝 黄芩 体内 上睑下垂 非酒精性脂肪肝 脂肪变性 药理学 化学 纤维化 肝损伤 基因敲除 炎症 生物化学 生物 炎症体 医学 免疫学 病理 内分泌学 疾病 中医药 细胞凋亡 高效液相色谱法 替代医学 生物技术 色谱法
作者
Huilian Shi,Fei Qiao,Weiting Lu,Kaiyue Huang,Yuanyuan Wen,Lifang Ye,Yuanyuan Chen
出处
期刊:European Journal of Pharmacology [Elsevier]
卷期号:934: 175270-175270 被引量:22
标识
DOI:10.1016/j.ejphar.2022.175270
摘要

Being at the important pathological stage and the critical treatment period of non-alcoholic fatty liver disease (NAFLD), nonalcoholic steatohepatitis (NASH) which is associated with fibrosis, hepatic and liver cancer has become a serious medical problem. As one of the major effective components in Scutellaria baicalensis, baicalin takes on anti-oxidant and anti-inflammatory activities. Nevertheless, its effects on NASH and its underlying molecular mechanism have not been thoroughly understood yet. In previous study, we have clarified baicalin could inhibit pyroptosis of hepatocytes mediated by NLRP3 in vitro, but the verification in vivo and upstream mechanism still need further work. Here the NASH mouse model was induced by feeding with a high fat diet (HFD) for 8-12 weeks. Thereafter, in the following weeks, NASH mice were given with HFD plus baicalin. We, subsequently, examined their hepatic function and inflammatory response and conducted the HE staining of liver samples. Furthermore, the underlying molecular mechanism was revealed through diverse molecular biological experiments including quantitative real-time PCR (qRT-PCR), Western blotting (WB), siRNA and CCK8 assays in HepG2 cells incubated with free fatty acid, and was verified in NASH mice. The in vivo findings indicated that baicalin decreased lipid accumulation and inflammation in the liver tissues of NASH mice, as evidenced by the enhanced NRF2/HO-1 expression and the reduced NLRP3/Caspase1/GSDMD levels, and these factors were involved in the pyroptosis pathway. Meanwhile, baicalin also contributed greatly against oxidative injury. The anti-inflammatory effect of baicalin was confirmed by experiments in vitro. For another, knockdown of NRF2 obviously weakened the protective effects of baicalin and reduced the NLRP3/Caspase1/GSDMD-mediated pyroptosis. This study indicates that baicalin is able to attenuate hepatic cell pyroptosis in vivo and in vitro in the case of NASH by regulating the NRF2/HO-1/NRLP3 pathway.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
畅快新之完成签到,获得积分10
1秒前
1111颂完成签到,获得积分10
2秒前
Owen应助木子采纳,获得10
3秒前
songyu发布了新的文献求助10
3秒前
xiaoxiao完成签到,获得积分10
4秒前
无限的石头完成签到 ,获得积分10
7秒前
muzi完成签到,获得积分10
7秒前
ZJFL完成签到,获得积分10
8秒前
8秒前
科目三应助顺心书琴采纳,获得10
8秒前
9秒前
上好发布了新的文献求助10
9秒前
迷路的舞蹈完成签到 ,获得积分10
10秒前
10秒前
FashionBoy应助萌123采纳,获得10
10秒前
任性向日葵完成签到,获得积分20
11秒前
852应助MJN采纳,获得10
11秒前
无足鸟发布了新的文献求助10
12秒前
无足鸟发布了新的文献求助10
12秒前
无足鸟发布了新的文献求助10
12秒前
fareless完成签到 ,获得积分10
12秒前
来勺麦旋风完成签到,获得积分10
12秒前
NexusExplorer应助007采纳,获得10
13秒前
13秒前
无足鸟发布了新的文献求助10
13秒前
15秒前
酷波er应助Alisa采纳,获得10
15秒前
16秒前
梁家小卖部完成签到,获得积分10
17秒前
WYX发布了新的文献求助10
18秒前
20秒前
20秒前
LL来了完成签到 ,获得积分10
20秒前
安静的飞珍完成签到,获得积分10
22秒前
23秒前
23秒前
25秒前
25秒前
Anata完成签到,获得积分10
26秒前
27秒前
高分求助中
Sustainability in Tides Chemistry 2000
Bayesian Models of Cognition:Reverse Engineering the Mind 800
Essentials of thematic analysis 700
A Dissection Guide & Atlas to the Rabbit 600
Very-high-order BVD Schemes Using β-variable THINC Method 568
A Photographic Guide to Mantis of China 常见螳螂野外识别手册 500
Natural History of Mantodea 螳螂的自然史 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3124684
求助须知:如何正确求助?哪些是违规求助? 2775048
关于积分的说明 7725009
捐赠科研通 2430539
什么是DOI,文献DOI怎么找? 1291201
科研通“疑难数据库(出版商)”最低求助积分说明 622091
版权声明 600323