碳纳米管
纳米生物技术
纳米技术
生物分子
材料科学
红外线的
生物电子学
多细胞生物
生物物理学
生物传感器
化学
纳米颗粒
细胞
生物
光学
物理
生物化学
作者
Alessandra Antonucci,Melania Reggente,Charlotte Roullier,Alice J. Gillen,Nils Schuergers,Vitālijs Zubkovs,Benjamin Lambert,Mohammed Mouhib,Elisabetta Carata,Luciana Dini,Ardemis A. Boghossian
标识
DOI:10.1038/s41565-022-01198-x
摘要
The distinctive properties of single-walled carbon nanotubes (SWCNTs) have inspired the development of many novel applications in the field of cell nanobiotechnology. However, studies thus far have not explored the effect of SWCNT functionalization on transport across the cell walls of prokaryotes. We explore the uptake of SWCNTs in Gram-negative cyanobacteria and demonstrate a passive length-dependent and selective internalization of SWCNTs decorated with positively charged biomolecules. We show that lysozyme-coated SWCNTs spontaneously penetrate the cell walls of a unicellular strain and a multicellular strain. A custom-built spinning-disc confocal microscope was used to image the distinct near-infrared SWCNT fluorescence within the autofluorescent cells, revealing a highly inhomogeneous distribution of SWCNTs. Real-time near-infrared monitoring of cell growth and division reveal that the SWCNTs are inherited by daughter cells. Moreover, these nanobionic living cells retained photosynthetic activity and showed an improved photo-exoelectrogenicity when incorporated into bioelectrochemical devices.
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