Batch and continuous flow asymmetric synthesis of anabolic-androgenic steroids via a single-cell biocatalytic Δ1-dehydrogenation and C17β-carbonyl reduction cascade

Asymmetric synthesis of (+)-boldenone and (+)-boldenone undecylenate was achieved in batch and continuous flow, using an enzyme cascade process catalyzed by an engineered Δ 1 -KstD and a mined 17β-CR.