圆二色性
化学
脂肪酶
蛋白质二级结构
吸附
猝灭(荧光)
生物物理学
构象变化
荧光
色氨酸
酪氨酸
活动站点
胰脂肪酶
光化学
生物化学
酶
有机化学
物理
生物
氨基酸
量子力学
作者
Ben Yu,Qingmiao Tang,Caixia Fu,Joe M. Regenstein,Junyi Huang,Lufeng Wang
出处
期刊:Food Chemistry
[Elsevier]
日期:2022-08-01
卷期号:398: 133834-133834
被引量:38
标识
DOI:10.1016/j.foodchem.2022.133834
摘要
Insoluble dietary fibre from citrus peels (CIDF) was found to have adsorption and inhibitory effect on the activity of pancreatic lipase (PL). CIDF-400 exhibited the greatest adsorption and activity inhibition effect on PL. The fluorescence quenching spectra indicated that CIDF could quench PL through a dynamic quenching process induced by the electrostatic interactions with only one binding site between them. The synchronous fluorescence and three-dimensional fluorescence spectra showed that CIDF might combine with PL to induce the increase in hydrophobicity and the reduction in polarity of tyrosine (Tyr) and tryptophan (Try) residues, which further led to the conformational alternations of PL. Moreover, circular dichroism (CD) showed that CIDF altered the secondary structure of PL, decreased α-helical structure content, and increased β-sheet structure content, potentially resulting in PL structure opening and its active site exposure. This study provides new perspectives for the application of CIDFs produced from agricultural waste in regulating lipid metabolism.
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