Differential aboveground-belowground adaptive strategies to alleviate N addition-induced P deficiency in two alpine coniferous forests

Increasing atmospheric nitrogen (N) deposition has resulted in phosphorus (P) limitation in multiple terrestrial ecosystems, yet how plants coordinate aboveground and belowground strategies to adapt to such P deficiency remains unclear. In this study, we conducted a field N fertilization experiment in two alpine coniferous plantations (Picea asperata Mast. and Pinus armandii Franch.) with different soil N availability on the eastern Tibetan Plateau of China, to examine N addition effects on plant nutrient limiting status and plant adaptive strategies corresponding to aboveground P conservation and belowground P acquisition. The results showed that N addition aggravated P deficiency in both plantations, as indicated by decreased needle P concentrations and increased N:P ratios, and that plant strategies for addressing such P deficiency differed in the two plantations with different initial soil N availabilities. In the P. asperata plantation with relatively high N availability, significantly enhanced needle phosphatase activity and shifts in P fraction allocation (downregulation of the structural P fraction and increased allocation to the residual P fraction) co-occurred with increased rhizosphere effects on phosphatase activity under N addition, indicating a synergistic strategy of aboveground P conservation and belowground P mining to alleviate P deficiency. In the P. armandii plantation with relatively low N availability, however, N addition only enhanced phosphatase activity and increased allocation to residual P fraction in the aboveground but had little effect on belowground P acquisition-associated traits, suggesting a decoupling relationship between aboveground P conservation and belowground P acquisition. This study highlights the vital significance of initial soil nutrient availability in regulating the coordination of aboveground and belowground strategic alternatives, emphasizing the need to integrate soil nutrient conditions for a holistic understanding of forest adaptation to anthropogenic N enrichment.