Bacterial community response in ginseng rhizosphere soil after Pseudomonas P1 inoculation integrating intracellular non-targeted metabolomics analysis

根际 代谢组学 荧光假单胞菌 人参 磷酸盐 假单胞菌 微生物种群生物学 生物 接种 食品科学 化学 细胞外 微生物学 生物化学 细菌 园艺 色谱法 有机化学 遗传学 替代医学 病理 医学
作者
Hai Sun,Cai Shao,Hao Liang,Jiaqi Qian,Qiao Jin,Jiapeng Zhu,Guojia Zhang,Bochen Lv,Yayu Zhang
出处
期刊:Environmental Technology and Innovation [Elsevier BV]
卷期号:35: 103633-103633 被引量:3
标识
DOI:10.1016/j.eti.2024.103633
摘要

To overcome the problems associated with insufficient available phosphorus in ginseng-growing soil, non-targeted metabolomics and high-throughput sequencing were adopted in this study to reveal the intracellular metabolites of Pseudomonas P1 and their effects on the soil bacterial community structure in ginseng-growing soil after Pseudomonas P1 inoculation. The results showed that although the P1 colony number was 4.68×107 cfu/mL after 32 h of cultivation, the available phosphorus stabilized at 24 h. Furthermore, 351 differential metabolites (162 and 189 in the positive and negative ion models, respectively) were screened from the eight P1 cultivation groups. Venn diagrams revealed more common metabolites in the negative ion model (45 metabolites) than in the positive ion model (17 metabolites). Organic acids and derivatives, at 23.53% and 15.56% in the positive- and negative-ion models, respectively, may be the dominant metabolites involved in phosphate dissolution. Although P1 inoculation had little effect on bacterial diversity, significant changes in bacterial phyla composition were identified between P10 and CK. Specifically, the relative abundance of Pseudomonadales in P10 was higher than that in CK (P < 0.05). Canonical discriminant analyses and correlation analysis showed that the shifts in bacterial community composition and structure were closely related to physicochemical factors, such as available phosphate, total potassium, NH4+-N, NO3--N, and pH. Our results suggest that P1 intracellular metabolites contain several organic acids, which reduce soil pH after inoculation, affect the supply of available phosphate, and change the composition of the microbial community structure and direction of cluster evolution.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
YouziBa完成签到,获得积分10
刚刚
萧然完成签到,获得积分10
刚刚
1秒前
1秒前
JamesPei应助研友_892kOL采纳,获得10
2秒前
科奇应助喜洋洋采纳,获得10
4秒前
月亮褪色了完成签到 ,获得积分10
4秒前
淡然水绿完成签到,获得积分10
5秒前
一路硕博完成签到,获得积分10
6秒前
李李李完成签到,获得积分10
8秒前
Owen应助王月缶采纳,获得10
8秒前
野草完成签到,获得积分10
8秒前
神勇千万完成签到,获得积分10
10秒前
...完成签到 ,获得积分0
12秒前
小雯完成签到,获得积分10
14秒前
任性的思远完成签到 ,获得积分10
15秒前
忘崽子小拳头完成签到,获得积分10
15秒前
15秒前
Hello应助by采纳,获得10
17秒前
友好傲白完成签到,获得积分10
18秒前
jianglili完成签到,获得积分10
19秒前
快乐的纸飞机完成签到 ,获得积分10
19秒前
二二完成签到 ,获得积分10
19秒前
叫我陈老师啊完成签到,获得积分10
20秒前
LILI完成签到,获得积分10
21秒前
亚亚完成签到 ,获得积分10
21秒前
种花兔完成签到,获得积分10
21秒前
桐桐应助Wang采纳,获得10
23秒前
仲大船完成签到,获得积分10
23秒前
今天进步了吗完成签到,获得积分10
24秒前
之诚关注了科研通微信公众号
25秒前
谢尔顿完成签到,获得积分10
25秒前
25秒前
肥逗完成签到 ,获得积分10
25秒前
lllllllll完成签到,获得积分10
26秒前
等待的谷波完成签到 ,获得积分10
27秒前
Singularity完成签到,获得积分0
27秒前
29秒前
29秒前
王月缶发布了新的文献求助10
29秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
Immigrant Incorporation in East Asian Democracies 600
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3968578
求助须知:如何正确求助?哪些是违规求助? 3513406
关于积分的说明 11167631
捐赠科研通 3248853
什么是DOI,文献DOI怎么找? 1794499
邀请新用户注册赠送积分活动 875150
科研通“疑难数据库(出版商)”最低求助积分说明 804671