Bacterial community response in ginseng rhizosphere soil after Pseudomonas P1 inoculation integrating intracellular non-targeted metabolomics analysis

根际 代谢组学 荧光假单胞菌 人参 磷酸盐 假单胞菌 微生物种群生物学 生物 接种 食品科学 化学 细胞外 微生物学 生物化学 细菌 园艺 色谱法 有机化学 遗传学 替代医学 病理 医学
作者
Hai Sun,Cai Shao,Hao Liang,Jiaqi Qian,Qiao Jin,Jiapeng Zhu,Guojia Zhang,Bochen Lv,Yayu Zhang
出处
期刊:Environmental Technology and Innovation [Elsevier BV]
卷期号:35: 103633-103633 被引量:3
标识
DOI:10.1016/j.eti.2024.103633
摘要

To overcome the problems associated with insufficient available phosphorus in ginseng-growing soil, non-targeted metabolomics and high-throughput sequencing were adopted in this study to reveal the intracellular metabolites of Pseudomonas P1 and their effects on the soil bacterial community structure in ginseng-growing soil after Pseudomonas P1 inoculation. The results showed that although the P1 colony number was 4.68×107 cfu/mL after 32 h of cultivation, the available phosphorus stabilized at 24 h. Furthermore, 351 differential metabolites (162 and 189 in the positive and negative ion models, respectively) were screened from the eight P1 cultivation groups. Venn diagrams revealed more common metabolites in the negative ion model (45 metabolites) than in the positive ion model (17 metabolites). Organic acids and derivatives, at 23.53% and 15.56% in the positive- and negative-ion models, respectively, may be the dominant metabolites involved in phosphate dissolution. Although P1 inoculation had little effect on bacterial diversity, significant changes in bacterial phyla composition were identified between P10 and CK. Specifically, the relative abundance of Pseudomonadales in P10 was higher than that in CK (P < 0.05). Canonical discriminant analyses and correlation analysis showed that the shifts in bacterial community composition and structure were closely related to physicochemical factors, such as available phosphate, total potassium, NH4+-N, NO3--N, and pH. Our results suggest that P1 intracellular metabolites contain several organic acids, which reduce soil pH after inoculation, affect the supply of available phosphate, and change the composition of the microbial community structure and direction of cluster evolution.
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