细胞毒性T细胞
巨噬细胞极化
免疫系统
体外
流式细胞术
效应器
重编程
巨噬细胞
表型
体内
癌症研究
生物
癌症
癌细胞
免疫学
细胞
生物化学
遗传学
基因
作者
Aldo Ummarino,Clément Anfray,Akihiro Maeda,Fernando Torres,Paola Allavena
出处
期刊:Methods in molecular biology
日期:2023-01-01
卷期号:: 81-91
被引量:2
标识
DOI:10.1007/978-1-0716-2914-7_6
摘要
Tumor-associated macrophages (TAMs) play a key immunosuppressive role that limits the ability of the immune system to fight cancer and hinder the anti-tumoral efficacy of most treatments currently applied in the clinic. However, a key feature of macrophages is their phenotypical and functional plasticity, which called their attention as promising targets for therapeutic intervention based on their elimination or reprogramming toward M1-like cytotoxic effector cells, with anti-tumor functions. This polarization status of macrophages can be studied in terms of molecular markers and functional activities, using an appropriate combination of experimental methodologies, both in vitro and in vivo. Here we focus on describing in vitro protocols to isolate primary monocytes from buffy coats and to study macrophage phenotype and function, after exposure to new therapies, by a combination of flow cytometry, RT-PCR, and ELISA analysis. We also provide the methodology to evaluate in vitro the cytotoxic activity of treated macrophages toward cancer cells.
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