Specific expression network analysis of diabetic nephropathy kidney tissue revealed key methylated sites

小桶 表观遗传学 基因 生物 遗传学 DNA甲基化 发病机制 基因表达 计算生物学 免疫学 转录组
作者
Yanzhe Wang,Wenwei Xu,Dingyu Zhu,Nan Zhang,Yonglan Wang,Miao Ding,Xinmiao Xie,Linlin Sun,Xiaoxia Wang
出处
期刊:Journal of Cellular Physiology [Wiley]
卷期号:233 (10): 7139-7147 被引量:23
标识
DOI:10.1002/jcp.26638
摘要

Diabetic nephropathy (DN) is one of the most common and serious complication in diabetes patients. However, the evidences of gene regulation mechanism and epigenetic modification with DN remain unclear. Therefore, it is necessary to search regulating genes for early diagnosis on DN. We identified tissue specific genes through mining the gene expression omnibus (GEO) public database, enriched function by gene ontology (GO), and kyoto encyclopedia of genes and genomes (KEGG) analysis, and further compared tissue-specific network. Meanwhile, combining with differentially methylated sites, we explored the association epigenetic modification with the pathogenesis of DN. Glomeruli (Glom) may be the main tissue of signal recognition and tubulointerstitium (Tub) is mainly associated with energy metabolism in the occurrence of DN. By comparing tissue-specific networks between Glom and Tub, we screened 319 genes, which played an important role in multiple tissue on kidney. Among them, ANXA2, UBE2L6, MME, IQGAP, SLC7A7, and PLG played a key role in regulating the incidence of DN. Besides, we also identified 1 up-regulated gene (PIK3C2B) and 39 down-regulated genes (POLR2G, DDB1, and ZNF230, etc.) in the methylated data of Glom specific genes. In the Tub specific expressed genes, we identified two hypo-methylated genes (PPARA and GLS). Tub mainly caused abnormal energy metabolism, and Glom caused the changes in cell connections and histone modification. By analyzing differentially methylated sites and tissue-specific expressed genes, we found the change of methylated status about the core regulating genes may be a potential factor in the pathogenesis of DN.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
复原乳完成签到,获得积分10
3秒前
公西寄凡发布了新的文献求助10
3秒前
4秒前
5秒前
wocao完成签到 ,获得积分10
5秒前
小蘑菇应助Dinse采纳,获得10
6秒前
maque4004完成签到,获得积分10
8秒前
皮老八发布了新的文献求助10
9秒前
李爱国应助xlogeman采纳,获得10
9秒前
9秒前
9秒前
dzll完成签到,获得积分10
10秒前
俞思含发布了新的文献求助10
12秒前
珈小羽完成签到,获得积分10
12秒前
13秒前
13秒前
13秒前
WNL完成签到,获得积分10
14秒前
15秒前
皮老八完成签到,获得积分10
15秒前
sayso发布了新的文献求助10
17秒前
哦耶zyy发布了新的文献求助10
18秒前
asd完成签到,获得积分20
18秒前
20秒前
20秒前
风起完成签到,获得积分10
20秒前
21秒前
21秒前
清汤不加盐完成签到,获得积分10
21秒前
22秒前
Danne应助尊敬惜萍采纳,获得10
23秒前
HOOKT完成签到,获得积分10
23秒前
23秒前
神勇的半莲完成签到,获得积分10
24秒前
风起发布了新的文献求助10
25秒前
26秒前
27秒前
onestepcloser完成签到 ,获得积分10
28秒前
28秒前
Lii开心发布了新的文献求助10
28秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
Evolution 3rd edition 1500
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 700
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 700
ALA生合成不全マウスでの糖代謝異常の分子機構解析 520
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3187833
求助须知:如何正确求助?哪些是违规求助? 2837552
关于积分的说明 8015738
捐赠科研通 2500230
什么是DOI,文献DOI怎么找? 1334789
科研通“疑难数据库(出版商)”最低求助积分说明 637296
邀请新用户注册赠送积分活动 605258