Use BAPN and AngII to establish SD rat aortic dissection model

Objective To establish the SD rat aortic dissection(AD) model by using both BAPN and AngⅡ, in order to investigate AD’s pathogenesis. Methods 90 three weeks old SD rats were equally divided into three groups randomly: control group, medicine gavage group and blank medicine gavage group. Rats in control group were fed on a regular diet; BAPN(1g/kg per day) was forced into rats’ stomach in the medicine gavage group; the same volume saline was forced into rats’ stomach in the blank medicine gavage group. 4 weeks later, when the rats were 7 weeks old, we stopped giving them BAPN, but to implant an omicro-osmotic pump subcutaneously in the abdomen. The pumps in control group and blank medicine gavage group were filled with 0.9% saline, the medicine gavage group’ pumps were filled with AngⅡsolution(1 μg·kg-1·min-1). 1 week later, all the survivals were dissected after anesthesia and the aortic vessels were acquired. All the acquired aortic vessels were proceed pathological examination. All the rats dead during the process of the experiment were dissected immediately to get the aortic vessels and proceed pathological examination. Results All rats in control group and blank medicine gavage group were survival, there was no aortic dissection or death. In medicine gavage group, 15 rats developped aortic dissection, 12 among them were died of aortic dissection rupture, the aortic dissection formation rate was 50%. Conclusion Using BAPN and AngⅡ to establish the SD rat AD model is feasible, it is simple and practicable, meanwhile, it has high aortic dissection formation rate. The process is similar with human’s aortic dissection process. Key words: BAPN; AngⅡ; Aortic dissection; Models, animal; Rats