Luteolin suppresses androgen receptor-positive triple-negative breast cancer cell proliferation and metastasis by epigenetic regulation of MMP9 expression via the AKT/mTOR signaling pathway

PI3K/AKT/mTOR通路 癌症研究 木犀草素 蛋白激酶B 雄激素受体 三阴性乳腺癌 雌激素受体 生物 乳腺癌 转移 细胞生长 信号转导 癌症 细胞生物学 前列腺癌 类黄酮 生物化学 遗传学 抗氧化剂
作者
Han‐Tsang Wu,Joseph Lin,Yi-En Liu,Hsiao‐Fan Chen,Kai‐Wen Hsu,Shu-Hsuan Lin,Kai‐Yen Peng,Kuo-Juei Lin,Chang‐Chi Hsieh,Dar‐Ren Chen
出处
期刊:Phytomedicine [Elsevier BV]
卷期号:81: 153437-153437 被引量:123
标识
DOI:10.1016/j.phymed.2020.153437
摘要

Triple-negative breast cancer (TNBC) represents up to 20% of all breast cancers. This cancer lacks the expression of the estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2. The current therapeutic strategy for patients with this subtype is the use of cytotoxic chemotherapy and surgery. Luteolin is a natural herbal flavonoid and a potential therapeutic candidate for multiple diseases. The use of a treatment that combines Chinese herbal medicine and western medicine is rising in Asia. The present study evaluates the effects and molecular mechanisms involved with luteolin treatment and evaluates whether this herb affects androgen receptor-positive breast cancer cell proliferation or metastasis. In vitro evaluation of the effect of luteolin on androgen receptor-positive TNBC cell proliferation and metastasis Cell viability analysis was used for the cytotoxicity test. Colony formation and Bromodeoxyuridine (BrdU) staining-based proliferation experiments were used for cell proliferation. Wound healing and transwell assays were used for in vitro migration/invasion. The RT-qPCR analysis was used for gene expression. Furthermore, ChIP-qPCR analysis was used for epigenetic modification of gene promoters. Luteolin significantly inhibited the proliferation and metastasis of androgen receptor-positive TNBC. Furthermore, luteolin inactivated the AKT/mTOR signaling pathway and reversed the epithelial-mesenchymal transition (EMT). The combination of luteolin and inhibitors of AKT/mTOR synergistically repressed an androgen receptor-positive TNBC cell proliferation and metastasis. Luteolin also downregulated MMP9 expression by decreasing the levels of the AKT/mTOR promoting H3K27Ac and H3K56A on the MMP9 promoter region. Our findings indicate that luteolin inhibited the proliferation and metastasis of androgen receptor-positive TNBC by regulating MMP9 expression through a reduction in the levels of AKT/mTOR-inducing H3K27Ac and H3K56Ac.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
华仔应助碧蓝碧凡采纳,获得10
刚刚
1秒前
超勍发布了新的文献求助10
5秒前
小马甲应助yuanshl1985采纳,获得10
5秒前
zhuxiaonian完成签到,获得积分10
8秒前
田様应助淘气科研采纳,获得10
8秒前
chenyi完成签到,获得积分10
9秒前
zyyyy完成签到,获得积分10
9秒前
奶黄包完成签到 ,获得积分10
9秒前
SYLH应助海阔天空采纳,获得10
9秒前
9秒前
机灵又蓝完成签到,获得积分10
10秒前
张土豆完成签到 ,获得积分10
10秒前
善学以致用应助小王采纳,获得10
10秒前
orang完成签到,获得积分10
11秒前
巧巧艾完成签到,获得积分10
11秒前
12秒前
邵洋完成签到,获得积分10
12秒前
sl发布了新的文献求助10
12秒前
13秒前
小迪迦奥特曼完成签到,获得积分10
13秒前
13秒前
cckk发布了新的文献求助10
14秒前
夏目由美完成签到 ,获得积分10
14秒前
Jasper应助哦哦哦采纳,获得10
15秒前
YYD完成签到,获得积分10
15秒前
超勍完成签到,获得积分10
15秒前
碧蓝碧凡发布了新的文献求助10
16秒前
Popeye应助鹤鸣采纳,获得30
16秒前
YYD发布了新的文献求助10
17秒前
yuanshl1985发布了新的文献求助10
17秒前
积极的黑猫完成签到,获得积分10
18秒前
GB完成签到 ,获得积分10
18秒前
Metx完成签到 ,获得积分10
19秒前
孤独的涔完成签到,获得积分10
20秒前
Jay完成签到,获得积分10
20秒前
21秒前
深情安青应助hf采纳,获得10
23秒前
学不懂数学应助大观天下采纳,获得10
23秒前
醉熏的水绿完成签到 ,获得积分10
23秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
Research on Disturbance Rejection Control Algorithm for Aerial Operation Robots 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038426
求助须知:如何正确求助?哪些是违规求助? 3576119
关于积分的说明 11374556
捐赠科研通 3305834
什么是DOI,文献DOI怎么找? 1819339
邀请新用户注册赠送积分活动 892678
科研通“疑难数据库(出版商)”最低求助积分说明 815029