显微镜
间隙
薄层荧光显微镜
光学
光学显微镜
显微镜
分辨率(逻辑)
材料科学
图像分辨率
相位成像
计算机科学
扫描电子显微镜
扫描共焦电子显微镜
物理
人工智能
泌尿科
医学
作者
Yanlu Chen,Xiaoliang Li,Dongdong Zhang,Chunhui Wang,Ruili Feng,Xuzhao Li,Yao Wen,Hao Xu,Xinyi Shirley Zhang,Xiao Yang,Yongyi Chen,Yi Feng,Bo Zhou,Bi-Chang Chen,Kai Y. Lei,Shanbao Cai,Jie–Min Jia,Liang Gao
出处
期刊:Cell Reports
[Elsevier]
日期:2020-11-01
卷期号:33 (5): 108349-108349
被引量:38
标识
DOI:10.1016/j.celrep.2020.108349
摘要
We present a tiling light sheet microscope compatible with all tissue clearing methods for rapid multicolor 3D imaging of cleared tissues with micron-scale (4 × 4 × 10 μm3) to submicron-scale (0.3 × 0.3 × 1 μm3) spatial resolution. The resolving ability is improved to sub-100 nm (70 × 70 × 200 nm3) via tissue expansion. The microscope uses tiling light sheets to achieve higher spatial resolution and better optical sectioning ability than conventional light sheet microscopes. The illumination light is phase modulated to adjust the position and intensity profile of the light sheet based on the desired spatial resolution and imaging speed and to keep the microscope aligned. The ability of the microscope to align via phase modulation alone also ensures its accuracy for multicolor 3D imaging and makes the microscope reliable and easy to operate. Here we describe the working principle and design of the microscope. We demonstrate its utility by imaging various cleared tissues.
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