Identification of Candida auris and related species by multiplex PCR based on unique GPI protein‐encoding genes

金念珠菌 基因 多重聚合酶链反应 生物 鉴定(生物学) 遗传学 聚合酶链反应 计算生物学 微生物学
作者
María Alvarado,Joaquín Bartolomé Álvarez,Shawn R. Lockhart,Eulogio Valentín,Alba Ruiz-Gaitán,Elena Eraso,Piet W. J. de Groot
出处
期刊:Mycoses [Wiley]
被引量:5
标识
DOI:10.1111/myc.13204
摘要

Background The pathogen Candida auris is rapidly gaining clinical importance because of its resistance to antifungal treatments and its persistence in hospital environments. Early and accurate diagnosis of C. auris infections is crucial, however, the fungus has often been misidentified by commercial systems. Objectives To develop conventional and real-time PCR methods for accurate and rapid identification of C. auris and its discrimination from closely related species by exploiting the uniqueness of certain glycosylphosphatidylinositol-modified protein-encoding genes. Methods Species-specific primers for two unique putative GPI protein-encoding genes per species were designed for C. auris, C. haemulonii, C. pseudohaemulonii, C. duobushaemulonii, C. lusitaniae, and C. albicans. Primers were blind tested for their specificity and efficiency in conventional and real-time multiplex PCR set-up. Results All primers combinations showed excellent species specificity. In multiplex mode, correct identification was aided by different sized amplicons for each species. Efficiency of the C. auris primers was validated using a panel of 155 C. auris isolates, including all known genetically diverse clades. In real-time multiplex PCR, different melting points of the amplicons allowed the distinction of C. auris from four related species. C. auris limit of detection was 5 CFU/reaction with a threshold value of 32. The method was also able to detect C. auris in spiked blood and serum. Conclusions PCR identification based on unique GPI protein-encoding genes allows for accurate and rapid species identification of C. auris and related species without need for expensive equipment when applied in conventional PCR set-up.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
无情夏寒完成签到 ,获得积分10
刚刚
慕青应助马士全采纳,获得10
1秒前
xuzj应助科研通管家采纳,获得10
1秒前
Rubby应助科研通管家采纳,获得30
2秒前
SciGPT应助科研通管家采纳,获得10
2秒前
2秒前
2秒前
shiizii应助科研通管家采纳,获得10
2秒前
2秒前
2秒前
2秒前
ludong_0应助科研通管家采纳,获得10
2秒前
YeeYee发布了新的文献求助10
2秒前
冷酷的松思完成签到,获得积分10
2秒前
zgt01发布了新的文献求助10
3秒前
zhang完成签到,获得积分10
3秒前
江中完成签到 ,获得积分10
5秒前
5秒前
阿玖完成签到 ,获得积分10
6秒前
jiaolulu发布了新的文献求助10
8秒前
踏雪飞鸿完成签到,获得积分10
9秒前
hannah完成签到,获得积分10
9秒前
songvv发布了新的文献求助10
10秒前
一一一应助Bin_Liu采纳,获得10
11秒前
麻果完成签到,获得积分10
13秒前
OER完成签到,获得积分10
13秒前
伦语完成签到,获得积分20
13秒前
中陆完成签到,获得积分10
14秒前
15秒前
莫西莫西完成签到,获得积分10
17秒前
19秒前
量子星尘发布了新的文献求助10
20秒前
xjh完成签到,获得积分10
20秒前
20秒前
lbnzd8g完成签到,获得积分10
22秒前
中海完成签到,获得积分10
22秒前
Ww完成签到,获得积分10
22秒前
伶俐不二完成签到,获得积分10
22秒前
XIAOJU_U完成签到 ,获得积分10
23秒前
马士全发布了新的文献求助10
24秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038201
求助须知:如何正确求助?哪些是违规求助? 3575940
关于积分的说明 11373987
捐赠科研通 3305747
什么是DOI,文献DOI怎么找? 1819274
邀请新用户注册赠送积分活动 892662
科研通“疑难数据库(出版商)”最低求助积分说明 815022