黑色素瘤
小RNA
中医药
医学
连环素
癌症研究
作用机理
污渍
Wnt信号通路
信号转导
药理学
生物
基因
病理
体外
遗传学
替代医学
作者
Yaping Wang,Xiu‐Qiong Fu,Chang-Cheng Yin,Ji‐Yao Chou,Yuxi Liu,Jing‐Xuan Bai,Ying‐Jie Chen,Ying Wu,Jia‐Ying Wu,Xiaoqi Wang,Bin Liu,Zhi‐Ling Yu
标识
DOI:10.1016/j.jep.2020.113065
摘要
Si-Jun-Zi-Tang (SJZT) is a traditional Chinese medicine formula used to treat chronic and debilitating diseases including melanoma. SJZT-based therapies have achieved good clinical outcomes in melanoma management. However, the pharmacological basis of SJZT for its clinical use in melanoma treatment is not fully understood.To investigate the anti-melanoma effects and mechanism of action of an ethanolic extract of SJZT.SJZT was extracted using 50% ethanol. A murine B16 melanoma-bearing mouse model was employed to investigate the anti-melanoma effects of SJZT. microRNA (miRNA) and mRNA levels were examined by RT-qPCR, and protein levels were measured by Western blotting.SJZT significantly inhibited B16 tumor growth in mice. Mechanistic investigations revealed that SJZT elevated miR-34b (a tumor suppressing miRNA), and lowered c-Met (a miR-34b target gene) and β-catenin (a downstream molecule of c-Met signaling) expression levels in the B16 tumors.In this study we found, for the first time, that SJZT exerts anti-melanoma effects and regulates the miR-34b/c-Met/β-catenin pathway in a melanoma mouse model. Our findings provide pharmacological justifications for the clinical use of SJZT in treating melanoma.
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