检出限
化学
分子印迹
荧光
木犀草素
色谱法
槲皮素
二醇
纳米传感器
线性范围
选择性
核化学
纳米技术
有机化学
材料科学
生物化学
催化作用
物理
抗氧化剂
量子力学
作者
Daojin Li,Simeng Zhai,Rumeng Song,Zheyao Liu,Weizhou Wang
标识
DOI:10.1016/j.saa.2019.117542
摘要
Novel boronate affinity imprinted quantum dots ([email protected] QDs) were used to develop a selective and sensitive fluorescent nanosensor for determination of cis-diol-containing flavonoids such as quercetin (Qu), baicalein (Bai) and luteolin (Lut) based on controllable oriented surface imprinting approach. The boronate affinity imprinted silica was used as recognition elements. Under the optimum conditions, the imprinting factor (IF) for Qu, Bai and Lut was evaluated to be 9.42, 6.58 and 10.91, respectively. The results indicated that the boronate affinity quantum dots coated with imprinted silica were successfully prepared. The obtained [email protected]MIPs QDs provided high selectivity and high sensitivity for cis-diol-containing flavonoids such as quercetin and luteolin. The [email protected] QDs exhibited linear decrease in fluorescence intensity with the increase of concentration of quercetin in the 0.05–25 μM concentration range. The detection limit (LOD) is evaluated to be 0.02 μM. The obtained fluorescent nanosensor could be successfully applied to efficient detection of cis-diol-containing flavonoids in onion skin and human urine samples. The recoveries for the spiked onion skin and urine samples were evaluated to be 83.50–104.00% and 86.67–105.00%, respectively. Clearly, this study provides a rapid and efficient fluorescent detection tool for cis-diol-containing flavonoids in real samples.
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