嗜酸性粒细胞过氧化物酶
CCL11型
脱颗粒
嗜酸性粒细胞
趋化性
嗜酸性粒细胞趋化因子
分泌物
嗜酸性阳离子蛋白
细胞生物学
刺激
化学
免疫学
生物
神经科学
生物化学
哮喘
受体
作者
Sergei I. Ochkur,Elizabeth A. Jacobsen,Paige Lacy
出处
期刊:Methods in molecular biology
日期:2021-01-01
卷期号:: 199-219
被引量:3
标识
DOI:10.1007/978-1-0716-1095-4_17
摘要
The analysis of eosinophil shape change and mediator secretion is a useful tool in understanding how eosinophils respond to immunological stimuli and chemotactic factors. Eosinophils undergo dramatic shape changes, along with secretion of the granule-derived enzyme eosinophil peroxidase (EPX) in response to chemotactic stimuli including platelet-activating factor (PAF) and CCL11 (eotaxin-1). Here, we describe the analysis of eosinophil shape change by confocal microscopy analysis and provide an experimental approach for comparing unstimulated cells with those that have been stimulated to undergo chemotaxis. In addition, we illustrate two different degranulation assays for EPX using OPD and an ELISA technique and show how eosinophil degranulation may be assessed from in vitro as well as ex vivo stimulation.
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