Preparation and use of wireless reprogrammable multilateral optogenetic devices for behavioral neuroscience
光遗传学
计算机科学
无线
神经科学
电信
生物
作者
Yiyuan Yang,Mingzheng Wu,Amy J. Wegener,Abraham Vázquez‐Guardado,Andrew I. Efimov,Ferrona Lie,Taoyi Wang,Yuhang Ma,Anthony Banks,Zhengwei Li,Zhaoqian Xie,Yonggang Huang,Cameron H. Good,Yevgenia Kozorovitskiy,John A. Rogers
Wireless battery-free optogenetic devices enable behavioral neuroscience studies in groups of animals with minimal interference to natural behavior. Real-time independent control of optogenetic stimulation through near-field communication dramatically expands the realm of applications of these devices in broad contexts of neuroscience research. Dissemination of these tools with advanced functionalities to the neuroscience community requires protocols for device manufacturing and experimental implementation. This protocol describes detailed procedures for fabrication, encapsulation and implantation of recently developed advanced wireless devices in head- and back-mounted forms. In addition, procedures for standard implementation of experimental systems in mice are provided. This protocol aims to facilitate the application of wireless optogenetic devices in advanced optogenetic experiments involving groups of freely moving rodents and complex environmental designs. The entire protocol lasts ~3–5 weeks. The authors present detailed procedures to fabricate, encapsulate and implant advanced wireless battery-free devices in head- and back-mounted forms in order to facilitate optogenetic experiments in freely moving rodents.