效应器
WRKY蛋白质结构域
拟南芥
生物
丁香假单胞菌
免疫受体
毒力
免疫系统
细胞生物学
计算生物学
微生物学
遗传学
病菌
基因
突变体
作者
Nitika Mukhi,Hannah Brown,Danylo Gorenkin,Pingtao Ding,Adam R. Bentham,Clare E. M. Stevenson,Jonathan D. G. Jones,Mark J. Banfield
标识
DOI:10.1073/pnas.2113996118
摘要
Significance This study reveals a mechanism for effector perception by a plant NLR immune receptor that contains an integrated domain (ID) that mimics an authentic effector target. The Arabidopsis immune receptors RRS1 and RPS4 detect the Pseudomonas syringae pv. pisi secreted effector AvrRps4 via a WRKY ID in RRS1. We used structural biology to reveal the mechanisms of AvrRps4 C –WRKY interaction and demonstrated that this binding is essential for effector recognition in planta. Our analysis revealed features of the WRKY ID that mediate perception of structurally distinct effectors from different bacterial pathogens. These insights could enable engineering NLRs with novel recognition specificities, and enhance our understanding of how effectors interact with host proteins to promote virulence.
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