Multiple Fingerprint Profiles and Chemometrics Analysis of Polysaccharides From the Roots of Glehnia littoralis

The quality of polysaccharides from different regions was studied by using multiple fingerprint analysis and chemometric analysis. Polysaccharides from 10 batches of Glehnia littoralis were compared based on Fourier-transform infrared spectroscopy (FT-IR), high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), and proton nuclear magnetic resonance ( 1 H-NMR). According to the results, the 10 batches of polysaccharides from G littoralis had high similarity by analyzing HPLC, FT-IR, 1 H-NMR, and GPC fingerprints. Through cluster analysis, samples and adulterants in different regions could be classified. Three monosaccharides (galactose, glucose, and galacturonic acid), molecular weights (4.33 × 10 5 -4.91 × 10 5 , 4.04 × 10 4 -4.71 × 10 4 , and 5.02 × 10 3 -5.83 × 10 3 ), and H-1 (4.99, 5.39, and 5.42 ppm) of α-glucose could be used as markers for quality control of medicinal materials of the roots of G littoralis.