Plasticity of T‐Cell Function

EL-4 lymphoma cells are able to suppress the primary humoral immune response of spleen cells to sheep erythrocytes or 4-hydroxy-3-iodo-5-nitrophenylacetyl-keyhole limpet haemocyanin (NIP-KLH) in vitro. Typically, the cells suppress very efficiently in small numbers, but lose this capacity as the numbers increase. Three clones were analysed and this fluctuation in function was paralleled by a fluctuation in the expression of CD8 (Ly-2). Clones were incubated for 2 days, starting from different seeding concentrations, and analysed with cytofluorography. Neither Thy-1 nor CD5 (Ly-1), H-2 K, H-2 D, LFA-1 (all positive) nor CD4 (L3T4) or MEL-14 (both negative) were influenced by this treatment. In contrast, cells were CD8- at high seeding concentrations, and CD8+ at low seeding concentrations. When cell cultures grew to higher densities, the cells again lost the capacity to express CD8. Experiments testing the suppressive capacity of individual EL-4 clones after preculture at different densities or in the presence of antibodies against CD8 suggest that the efficiency of suppression may well be correlated to the amount of CD8 expressed.