信号转导
蛋白激酶C
细胞迁移
下调和上调
NF-κB
佛波
p38丝裂原活化蛋白激酶
磷酸化
细胞生长
化学
基质金属蛋白酶
激酶
癌症研究
癌细胞
分子生物学
αBκ
细胞生物学
细胞
生物
癌症
MAPK/ERK通路
内科学
医学
生物化学
基因
作者
Sun-Hyung Ha,Kyung-Min Kwon,Jun-Young Park,Fukushi Abekura,Young Choon Lee,Tae‐Wook Chung,Ki‐Tae Ha,Hyeun Wook Chang,Seung-Hak Cho,Jong‐Suk Kim,Cheorl-Ho Kim
摘要
Abstract A water‐soluble saponin, Esculentoside H (EsH), 3‐ O ‐( O ‐β‐ d ‐glucopyranosyl‐(1→4)‐β‐ d ‐xylopyranosyl)‐28‐β‐ d ‐glucopyranosylphytolaccagenin has been isolated and purified from the root extract of perennial plant Phytolacca esculenta . EsH is known to be an anticancer compound, having a capacity for TNF‐α release. However, the effects of EsH on migration and growth in tumor cells have not yet been reported. In the current study, the suppressive effects of EsH on phorbol 12‐myristate 13‐acetate (PMA)‐induced cell migration were examined in murine colon cancer CT26 cells and human colon cancer HCT116 cells. Interestingly, the transwell assay and wound healing show that EsH suppresses the PMA‐induced migration and growth potential of HCT116 and CT26 colon cancer cells, respectively. EsH dose‐dependently suppressed matrix metalloproteinases‐9 (MMP‐9) expression that was upregulated upon PMA treatment in messenger RNA levels and protein secretion. Since the expression of MMP‐9 is correlated with nuclear factor‐κB (NF‐κB) signaling, it has been examined whether EsH inhibits PMA‐induced IκB phosphorylation that leads to the suppression of NK‐κB nuclear translocation. EsH repressed the phosphorylation level of JNK, but not extracellular signal‐regulated kinase and p38 signaling when the cells were treated with PMA. Overall, these results demonstrated that EsH could suppress cancer migration through blockage of the JNK1/2 and NF‐κB signaling‐mediated MMP‐9 expression.
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