间充质干细胞
祖细胞
生物
骨髓
祖细胞
细胞生物学
人口
啮齿动物
免疫学
干细胞
医学
生态学
环境卫生
作者
Ji Zhu,Valerie A. Siclari,Ling Qin
出处
期刊:Methods in molecular biology
日期:2014-09-17
卷期号:: 19-29
被引量:8
标识
DOI:10.1007/978-1-4939-1619-1_3
摘要
Bone marrow mesenchymal stem cells (MSCs) are promising therapeutic tools for tissue repair and the treatment of a number of human diseases. As a result, there is substantial interest in characterizing and expanding these cells to uncover their therapeutic potential. For preclinical studies, mesenchymal progenitors, containing both MSCs and their proliferative progeny, are commonly isolated from the central region of rodent long bones. However, challenges exist in expanding these central mesenchymal progenitors in culture. We have recently identified another population of progenitors within rodent long bones that resides close to the bone surface, which we termed endosteal mesenchymal progenitors. These cells are more metabolically active and more responsive to external stimuli compared to central mesenchymal progenitors and therefore, they represent a biologically important target for MSC research. This chapter describes in detail a unique enzymatic digestion approach to isolate and culture endosteal mesenchymal progenitors as well as their central counterparts from rodent long bones.
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