Leaf Gas Exchange and Water Relations of Lupins and Wheat. I. Shoot Responses to Soil Water Deficits

The effects of a progressive increase in soil water deficit on the leaf conductance and gas exchange of lupin (Lupinus cosentinii) and wheat (Triticum aestivum) were investigated in pot experiments in a temperature-regulated glasshouse, using a coarse, sandy soil characteristic of the Western Australian wheatbelt. Transpiration rates decreased rapidly in both species after water was withheld, mainly as a result of stomatal closure. Photosynthesis declined also, but to a lesser extent than conductance. Leaf extension in lupin was equally as sensitive to a decrease in leaf water potential and soil water potential as stomatal conductance. Stomatal closure served to maintain the water potential of lupin leaves to within 0.1 MPa of that of control (watered) plants as the soil water content decreased from 0.14 to 0.06 m3 m-3 and as the leaf conductance and the relative transpiration rate fell to less than 50% of control values. Maintenance of leaf water potential with decreasing soil water content and stomatal conductance was less evident in wheat. In both lupin and wheat, leaf conductance decreased linearly with soil water content and curvilinearly with bulk soil matric potential, indicating that water uptake was restricted at similar water contents and matric potentials in both species. Diurnal measurements on lupin indicated a substantial reduction in stomatal conductance after water was withheld, even when the leaf water potential at midday was reduced by only 0.1 MPa and no change could be detected in the bulk leaf turgor pressure. Conductance in lupin was reduced even though the soil matric potential decreased in only part of the rooting zone. This, together with the absence of any significant change in the leaf water potential, turgor pressure, or relative water content in lupin during the initial stages of stomatal closure, suggests that a soil or root factor initiates the reduction in leaf conductance - and hence regulates the shoot water status - in response to soil drying.