逆转录酶
底漆(化妆品)
生物
互补DNA
引物结合位点
碱基对
核糖核酸
分子生物学
DNA
核苷酸
RNA定向DNA聚合酶
遗传学
基因
化学
有机化学
作者
Bing Chen,Alan M. Lambowitz
标识
DOI:10.1006/jmbi.1997.1185
摘要
The Mauriceville mitochondrial retroplasmid of Neurospora encodes a novel reverse transcriptase that initiates cDNA synthesis at a 3′ tRNA-like structure of the plasmid transcript, either de novo (i.e. without a primer) or by using the 3′ OH group of a DNA primer. Both the de novo and primer-mediated initiations involve recognition of structural features at the 3′ end of the retroplasmid transcript, which ends with a 3′ CCACCA. Here, detailed biochemical characterization of the retroplasmid reverse transcriptase shows that the 3′ CCA of the plasmid transcript is the major structural feature recognized by the reverse transcriptase for both the de novo and primer-mediated initiations. Complementarity between the DNA primer and RNA template is not required for the primer-mediated initiation, although short (1 to 3 nt) base-pairing interactions can influence both the efficiency and site of initiation near the 3′ end of the transcript. Single nucleotide changes in the 3′ CCA lead to less efficient initiation in the upstream CCA with an increased propensity to add extra “non-coded” nucleotides to the 5′ end of the cDNA during de novo initiation or to the 3′ end of the primer during primer-mediated initiation. Secondary structure features upstream of the 3′ CCA also influence the efficiency of initiation, but are not stringently required in vitro. Finally, we find that the retroplasmid reverse transcriptase does not efficiently use DNA primers that are base-paired to internal positions in the RNA template, nor does it use analogs of natural substrates used by non-long terminal repeat retrotransposon or retroviral reverse transcriptases. Our results indicate that the retroplasmid reverse transcriptase is uniquely adapted to initiate cDNA synthesis by recognizing a 3′ CCA sequence. The ability to recognize a specific template sequence is common for RNA polymerases, but unprecedented for a reverse transcriptase.
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