延伸率
RNA聚合酶Ⅱ
转录泡
聚合酶
构象变化
过程性
核糖核酸
生物物理学
抄写(语言学)
生物
RNA聚合酶
DNA
化学
分子生物学
细胞生物学
RNA依赖性RNA聚合酶
生物化学
发起人
基因
基因表达
材料科学
语言学
哲学
冶金
极限抗拉强度
标识
DOI:10.1016/j.sbi.2004.01.006
摘要
The RNA polymerase from phage T7 is a 99 kDa single polypeptide that is unrelated to the multisubunit cellular RNA polymerases, but exhibits nearly all of their properties. Six separate crystal structures have enhanced our understanding of promoter DNA recognition, duplex DNA opening, and the transition from the abortive initiation phase to the elongation phase. A major conformational change in the N-terminal domain removes the promoter-binding site, accounting for promoter clearance, and creates a tunnel through which the transcript passes, accounting for the processivity of the elongation phase. Structures of substrate and product complexes show that a rotational conformational change of the fingers domain is associated with translocation and downstream strand separation. The rotation that results in translocation is powered by the release of the pyrophosphate product.
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