Cytokinin-like substances and ethylene production by Azospirillum in media with different carbon sources

Production of cytokinin-like substances (CLS) from two strains and ethylene from three strains of Azospirillum in the presence of different carbo sources was studied. Ethylene production depended on the presence of methionine and different carbon sources. CLS were detected by the soybean callus test and gas chromatography. A strong stimulation of soybean callus was stated with Azospirillum No 1 and 3 in media containing malate. In culture filtrates from both strains of Azospirillum 2iP = 6(γ,γ-dimethylallylamino)purine was detected. All strains of Azospirillum produced ethylene in the presence of methionine and malate, succinate and pyruvate.