Biomarkers for Prediction of Bovine Respiratory Disease Outcome

牛呼吸道疾病 结合珠蛋白 生物 代谢组学 疾病 生物标志物 免疫学 内科学 医学 生物信息学 生物化学
作者
Palok Aich,Lorne A. Babiuk,Andrew Potter,Philip Griebel
出处
期刊:Omics A Journal of Integrative Biology [Mary Ann Liebert]
卷期号:13 (3): 199-209 被引量:45
标识
DOI:10.1089/omi.2009.0012
摘要

Fatal bovine respiratory disease (BRD) is frequently the result of a primary viral and a secondary bacterial respiratory infection. In cattle, BRD causes more than half of feedlot deaths and has a major impact on financial losses in the cattle industry in North America. It is, therefore, very important to understand the mechanism of this complex disease process as well to predict and identify BRD susceptible cattle to enhance treatment efficacy. We recently established the value of using combinatorial omics approaches to identify candidate biomarkers associated with stress responses, a factor that can increase the severity of BRD. The objective of the present investigation was to experimentally recreate fatal BRD and to use a combinatorial analysis of proteomic, metabonomic, and elemental profiles in serum samples to determine if multimethod analysis of these biomarkers could predict disease outcome. The proteomic studies revealed that changes in the serum proteome were significant on day 4 postviral infection when compared to preinfection (day 0) serum samples. Proteomic studies identified a group of acute phase proteins (haptoglobin and apolipoprotein AI), which could be linked to a primary viral respiratory infection, but there was no significant association observed with fatal BRD. In contrast, metabonomic and elemental analyses identified candidate biomarkers for viral infection (glucose, LDL, valine, phosphorous, and iron) and disease outcome (lactate, glucose, iron). While multivariate analysis of proteomic and metabolite profiles did not discriminate between animals that survived or died postsynergic viral–bacterial infection by analyzing preinfection (day 0) serum samples, analysis of serum elemental profiles prior to infection was, however, predictive of BRD outcome. Furthermore, discriminant analyses of all three methodologies used to profile serum (collected on day 4 postviral but prior to bacterial infection) revealed differential trends between animals that survived or died following synergic viral–bacterial infection. Thus, a combinatorial approach using proteomic, metabonomic, and elemental analyses of serum samples revealed that multimethod analysis could discriminate between the complex biological responses to secondary bacterial respiratory infection and predict disease outcome.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
hhh完成签到,获得积分10
刚刚
顾矜应助和和采纳,获得10
1秒前
希望天下0贩的0应助曾珍采纳,获得10
2秒前
3秒前
1484发布了新的文献求助10
3秒前
许起眸应助奶油猫猫采纳,获得20
3秒前
很蓝的啦发布了新的文献求助20
4秒前
丽丽应助冷傲的白卉采纳,获得20
4秒前
mg完成签到,获得积分10
5秒前
要吃烧饼么完成签到,获得积分10
5秒前
Murphy_H完成签到,获得积分10
5秒前
无忧应助娟儿采纳,获得10
6秒前
yuu完成签到,获得积分10
8秒前
雨季发布了新的文献求助10
8秒前
9秒前
倾心红枫林完成签到,获得积分10
10秒前
hahhahahh发布了新的文献求助10
10秒前
小蘑菇应助nong12123采纳,获得10
10秒前
xiao发布了新的文献求助10
10秒前
赘婿应助星辰采纳,获得30
11秒前
大个应助无限静珊采纳,获得10
11秒前
今后应助Niraaaa采纳,获得10
13秒前
下水道修理工完成签到,获得积分10
13秒前
14秒前
Eavan完成签到,获得积分10
14秒前
贝壳发布了新的文献求助20
15秒前
汉堡包应助雨季采纳,获得10
15秒前
大聪明发布了新的文献求助10
15秒前
木林森发布了新的文献求助10
16秒前
16秒前
季宇完成签到,获得积分10
16秒前
16秒前
娟儿完成签到,获得积分10
17秒前
曾珍发布了新的文献求助10
17秒前
坚强的广山应助QQ采纳,获得200
19秒前
19秒前
19秒前
Elian发布了新的文献求助10
20秒前
尕辉发布了新的文献求助10
20秒前
彪壮的青亦完成签到,获得积分10
21秒前
高分求助中
Sustainability in Tides Chemistry 2000
The ACS Guide to Scholarly Communication 2000
Studien zur Ideengeschichte der Gesetzgebung 1000
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Pharmacogenomics: Applications to Patient Care, Third Edition 800
A Dissection Guide & Atlas to the Rabbit 500
Gerard de Lairesse : an artist between stage and studio 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3079288
求助须知:如何正确求助?哪些是违规求助? 2731907
关于积分的说明 7521504
捐赠科研通 2380646
什么是DOI,文献DOI怎么找? 1262460
科研通“疑难数据库(出版商)”最低求助积分说明 611947
版权声明 597414