牙周纤维
弹性蛋白酶
血小板源性生长因子受体
化学
细胞生物学
胰弹性蛋白酶
受体
流式细胞术
伤口愈合
蛋白质水解
磷酸化
分子生物学
免疫学
生物
生物化学
医学
生长因子
牙科
酶
作者
Eiji Nemoto,Sousuke Kanaya,Maiko Minamibuchi,Hidetoshi Shimauchi
标识
DOI:10.1177/154405910508400709
摘要
Human leukocyte elastase, a neutrophil serine protease, is considered to be a potential immunoregulatory protease. Since the PDGF receptor (PDGFR) on periodontal ligament (PDL) cells is a crucial element for various functions, such as wound healing in periodontal tissue, we investigated the effect of elastase on the expression of PDGFR on PDL cells by flow cytometry and Western blotting. We found that PDGFR-α disappeared with an increasing dose of elastase, and PDGFR-β was degraded into several fragments. Elastase degraded both receptors on fixed cells, indicating that the degradation resulted from direct proteolysis on the cell surface. Elastase also then disturbed the phosphorylation of ERK1/2, JNK/SARK, and p38, triggered by PDGF-AA and PDGF-BB, suggesting that elastase inhibited PDGFR-dependent cell activation in PDL cells. These results suggest that elastase may modulate the PDGF-mediated activity of PDL cells during periodontal wound healing.
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