壳聚糖
甲壳素
苏云金杆菌
基质(水族馆)
化学
低聚糖
食品科学
生物
生物化学
细菌
生态学
遗传学
作者
Dario Rafael Olicón‐Hernández,Pedro Alberto Vázquez‐Landaverde,Ramón Cruz‐Camarillo,Luz Irene Rojas Avelizapa
标识
DOI:10.1080/10826068.2016.1181086
摘要
Bacillus thuringiensis is a nonhuman pathogen bacterium that is used as a fungal and insect biocontrol agent. Because of its environmental interaction, it possesses several extracellular enzymes that are able to degrade chitin and chitosan, two of the most important polymers because of their application in numerous fields. However, in recent years, it has been observed that oligosaccharides from the enzymatic degradation of chitosan have important benefits for human health. Comparison and exploration of the production of chito-oligosaccharides from different sources of chitosan will improve the process parameters and expand the biotechnology based in these molecules. This study shows the production of chito-oligosaccharides from three different sources of colloidal chitosan and conducts a qualitative–quantitative comparison between them, using the extracellular enzyme of B. thuringiensis. We found that in the three substrates, it is possible to get a mixture of chito-oligosaccharides from dimer to hexamer in a concentration range from 0.72 to 8.09 mg · g−1 of original substrate. The best substrate to obtain these molecules was commercial chitosan as it has the highest production yields.
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