Nanoparticle based amperometric biosensor for the quantitative determination of cholesterol in human blood

Total cholesterol monitoring in human blood serum is one of the most important routine analysis performed in clinical laboratory.Epidemiological studies have shown a strong correlation between coronary heart disease and blood cholesterol level.There is need for a method that is sufficiently flexible to yield good results in clinical laboratory.Numbers of cholesterol biosensors have been developed over the past 30 years.Fibre-optic fluorescence, Fibre-optic luminescence, Potentiometric, Spectrophotometric and Fluorometric biosensors, which determine cholesterol enzymatically.Some of these methods suffer from interference from other substances found in the blood such as ascorbic acid and uric acid.Therefore amperometric biosensor was designed based on titanium oxide nano particle with Advanced RISC (Reduced Instruction Set Computing) Machine processor to determine the cholesterol level in human blood.