内分泌学
内科学
链脲佐菌素
尿道
医学
糖尿病
胰岛素
泌尿系统
可溶性鸟苷酰环化酶
泌尿科
一氧化氮
鸟苷酸环化酶
作者
Daisuke Gotoh,Nailong Cao,Eduardo Costa Alexandre,Tetsuichi Saito,Yosuke Morizawa,Shunta Hori,Makito Miyake,Kazumasa Torimoto,Kiyohide Fujimoto,Naoki Yoshimura
出处
期刊:Life Sciences
[Elsevier]
日期:2021-12-01
卷期号:286: 120001-120001
被引量:5
标识
DOI:10.1016/j.lfs.2021.120001
摘要
To examine the effects of low-dose insulin or a soluble guanylate cyclase activator (sGC) on lower urinary tract dysfunction (LUTD) in rats with diabetes mellitus (DM).Female Sprague-Dawley rats were divided into non-DM control (N), DM induced by streptozotocin (65 mg/kg), with low-dose insulin (DI), DM with vehicle (D), and DM with sGC (GC) groups. In GC group, BAY 60-2770 (1 mg/kg/day) was orally administered in 6-8 weeks after DM. Voiding assay at 2, 4, and 8 weeks after DM, cystometry, and urethral pressure recordings at 8 weeks of DM were performed. mRNA levels of NO-related markers and cGMP protein levels in the urethra, and ischemia and inflammation markers in the bladder were evaluated by RT-PCR.Moderate levels of high blood glucose were maintained in Group DI versus Group D. The 24-h voided volume was significantly higher in Group D versus Groups N and DI. Non-voiding contractions were significantly greater, and voiding efficiency and urethral pressure reduction were significantly lower in Group D versus Groups N, DI, and GC. Urethral cGMP levels were significantly lower in Group D versus Groups N and GC. mRNA levels of PDE5 in the urethra and ischemia and inflammation markers in the bladder increased in Group D versus Group N or DI was reduced after sGC treatment.DI rats with a lesser degree of bladder and urethral dysfunction might be useful as a slow-progressive DM model. sGC activation could be an effective treatment of LUTD in DM.
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