核梭杆菌
生物
梭杆菌门
基因
核糖核酸
计算生物学
遗传学
基因表达
平铺阵列
转录组
细菌
16S核糖体RNA
牙龈卟啉单胞菌
拟杆菌
作者
Falk Ponath,Caroline Tawk,Yan Zhu,Lars Barquist,Franziska Faber,Jörg Vogel
出处
期刊:Nature microbiology
日期:2021-07-08
卷期号:6 (8): 1007-1020
被引量:36
标识
DOI:10.1038/s41564-021-00927-7
摘要
Fusobacterium nucleatum, long known as a constituent of the oral microflora, has recently garnered renewed attention for its association with several different human cancers. The growing interest in this emerging cancer-associated bacterium contrasts with a paucity of knowledge about its basic gene expression features and physiological responses. As fusobacteria lack all established small RNA-associated proteins, post-transcriptional networks in these bacteria are also unknown. In the present study, using differential RNA-sequencing, we generate high-resolution global RNA maps for five clinically relevant fusobacterial strains-F. nucleatum subspecies nucleatum, animalis, polymorphum and vincentii, as well as F. periodonticum-for early, mid-exponential growth and early stationary phase. These data are made available in an online browser, and we use these to uncover fundamental aspects of fusobacterial gene expression architecture and a suite of non-coding RNAs. Developing a vector for functional analysis of fusobacterial genes, we discover a conserved fusobacterial oxygen-induced small RNA, FoxI, which serves as a post-transcriptional repressor of the major outer membrane porin FomA. Our findings provide a crucial step towards delineating the regulatory networks enabling F. nucleatum adaptation to different environments, which may elucidate how these bacteria colonize different compartments of the human body.
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