Development of autotrophic and heterotrophic consortia via immobilized microbial beads for chemical wastewater treatment, using PTA wastewater as an approach

Studies on the symbiosis of microalgae-bacteria have been accelerating as a mean for wastewater remediation. However, there were few reports about the microalgae-bacteria consortia for chemical wastewater treatment. The aim of the present study is to develop an autotrophic and heterotrophic consortium for chemical wastewater treatment and probe whether and how bacteria could benefit from the microalgae during the treatment process, using PTA wastewater as an approach. A process-dependent strategy was applied. First of all, the results showed that the sludge beads with the sludge concentration of 30 g/L were the optimal one with the COD removal rate at 84.8% but the ceiling effect occurred (COD removal rate < 90%) even several common reinforcement methods were applied. Additionally, by adding the microalgae Chlorella vulgaris, a microalgae-activated sludge consortium was formed inside the immobilized beads, which provided better performance to shatter the ceiling effect. The COD remove rate was higher than 90%, regardless of the activated sludge was pre-culture or not. COD removal capacity could also be improved (COD removal rate > 92%) when LEDs light belt was offered as an advanced light condition. Biochemical assay and DNA analysis indicated that the microalgae could form an internal circulation of substances within the activated sludge and drove the microbial community to success and the corresponding gene functions, like metabolism and.