The ABA receptor gene MdPYL9 confers tolerance to drought stress in transgenic apple (Malus domestica)

脱落酸 生物 苹果属植物 拟南芥 转基因 耐旱性 基因 非生物胁迫 转基因作物 基因家族 植物 基因表达 生物化学 突变体
作者
Jie Yang,Min Wang,Shasha Zhou,Bingyao Xu,Peihong Chen,Fengwang Ma,Ke Mao
出处
期刊:Environmental and Experimental Botany [Elsevier]
卷期号:194: 104695-104695 被引量:25
标识
DOI:10.1016/j.envexpbot.2021.104695
摘要

Abscisic acid (ABA) is an important phytohormone for plant growth, development, and the response to various types of abiotic stress. Pyrabactin Resistance 1 (PYR1)/PYR1-Like (PYL)/Regulatory Component of ABA Receptor (RCAR) family proteins (PYLs) are ABA receptors that have been studied in many plant species. However, no detailed functional studies of these genes in regulating apple drought tolerance have been reported. Here, we isolated a homolog of the ABA receptor PYL9 in Arabidopsis, MdPYL9, from apple (Malus domestica) and analyzed its role in regulating drought tolerance in transgenic apple plants. Gene structure and protein sequence analysis showed that MdPYL9 shares a high similarity with AtPYL9. Protein sequence comparison and 3D structure prediction identified several conserved motifs and residues that are important for ABA binding in MdPYL9, including the helix-grip structure and the gate and latch loops. Expression analysis showed that MdPYL9 was significantly induced by drought treatment. Overexpression of MdPYL9 conferred enhanced tolerance to drought stress in transgenic apple plants, including a higher photosynthetic rate and relative water content and lower MDA content and electrolyte leakage compared with wild-type plants. Lower accumulation of reactive oxygen species (ROS) and higher activity of antioxidant enzymes suggest that MdPYL9 overexpression could promote ROS scavenging and thus reduce oxidative damage in transgenic apple plants under drought stress. Yeast two-hybrid assays indicated that there were both ABA-dependent and ABA-independent interactions between MdPYL9 and the clade A subgroup members of the PP2C family (PP2CAs) in apple. Expression analysis identifed two PP2CA genes, MdHAI1 and MdHAI2, that were highly expressed under drought treatment by not expressed under normal conditions. These results provide new insight that could be used to aid future studies examining the function of MdPYLs and the mechanism by which they regulate apple drought tolerance.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
Hello应助橘子味的风采纳,获得10
2秒前
寒冷的慕灵完成签到,获得积分10
2秒前
3秒前
激动的猫咪完成签到,获得积分10
3秒前
一只废鼠发布了新的文献求助10
3秒前
等待诗柳完成签到,获得积分10
3秒前
找文献啊找文献应助面包采纳,获得10
4秒前
善学以致用应助bigstone采纳,获得10
4秒前
5秒前
6秒前
serein发布了新的文献求助10
6秒前
科研猫发布了新的文献求助10
6秒前
6秒前
7秒前
善学以致用应助北极星采纳,获得10
8秒前
10秒前
霜沐发布了新的文献求助30
10秒前
无花果应助橘子味的风采纳,获得10
11秒前
wwdd完成签到,获得积分10
11秒前
寻雪完成签到,获得积分10
11秒前
12秒前
ssss发布了新的文献求助30
12秒前
雅青完成签到 ,获得积分10
12秒前
15秒前
倦梦还完成签到,获得积分10
15秒前
15秒前
情怀应助自由的水卉采纳,获得10
15秒前
落后月亮发布了新的文献求助10
16秒前
deer发布了新的文献求助10
16秒前
香蕉觅云应助木头采纳,获得10
16秒前
17秒前
18秒前
18秒前
一口一个小朋友完成签到,获得积分10
19秒前
19秒前
英姑应助lvsehx采纳,获得10
20秒前
星辰大海应助可英采纳,获得10
21秒前
21秒前
隐形曼青应助橘子味的风采纳,获得10
22秒前
高分求助中
求国内可以测试或购买Loschmidt cell(或相同原理器件)的机构信息 1000
The Heath Anthology of American Literature: Early Nineteenth Century 1800 - 1865 Vol. B 500
A new species of Velataspis (Hemiptera Coccoidea Diaspididae) from tea in Assam 500
Sarcolestes leedsi Lydekker, an ankylosaurian dinosaur from the Middle Jurassic of England 500
Machine Learning for Polymer Informatics 500
《关于整治突出dupin问题的实施意见》(厅字〔2019〕52号) 500
2024 Medicinal Chemistry Reviews 480
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3218909
求助须知:如何正确求助?哪些是违规求助? 2867929
关于积分的说明 8158830
捐赠科研通 2534996
什么是DOI,文献DOI怎么找? 1367373
科研通“疑难数据库(出版商)”最低求助积分说明 645033
邀请新用户注册赠送积分活动 618223