The Effects of Different Post-thawed Culture Periods on Clinical Outcomes in Frozen Embryo Transfer Cycle

The purpose of this study is to evaluate the effects of different post-thawed culture periods on the clinical outcomes. 9381 frozen embryo transfer (FET) cycles were divided into three groups according to female age: < 35, 35-39, and > 39 years, and two groups depending on post-thawed culture period before transfer: short culture (2-3 h) group (S) and long culture (18-20 h) group (L). According to the increment number of post-thawed embryos, the L group divided into three groups: ≤ 2, one ≤ 2 and the other > 2, and > 2 groups. Pregnancy outcomes included the implantation rate (IR), clinical pregnancy rate (CPR), multiple pregnancy rate (MPR), live birth rate (LBR), and neonatal characteristics. Long post-thawed culture caused a significant increase in the IR, CPR, MPR, and LBR (p = 0.000, 0.004, 0.037, and 0.001; CI = 1.06-1.194, 1.042-1.237, 1.008-1.254, and 1.054-1.245, respectively), and blastomere increment number also had a significant effect on IR, CPR, MPR, and LBR (p = 0.000, 0.000, 0.000, and 0.000, respectively). No significant differences were present in neonatal characteristics between the two post-thawed culture groups. Singleton group had a higher average gestational age and birthweight as well as a lower cesarean section rate, preterm labor rate, and low birthweight rate than twins group. Long post-thawed culture was associated with higher IR, CPR, MPR, and LBR, and transferring a well-developed embryo after long post-thawed culture might be a viable embryo transfer strategy to decrease MPR while maintaining CPR and LBR.