表位
肽
CD8型
单克隆抗体
免疫
分子生物学
T细胞
生物
抗原
免疫疗法
肽疫苗
免疫系统
流式细胞术
抗体
免疫学
病毒学
生物化学
作者
Hirohito Miyako,Yoshie Kametani,Ikumi Katano,Ryoji Ito,Banri Tsuda,Atsuko Furukawa,Yuki Saito,Dai Ishikawa,Kōichi Ogino,Shigeru Sasaki,Kohzoh Imai,Sonoko Habu,Hiroyasu Makuuchi,Yutaka Tokuda
出处
期刊:PubMed
日期:2011-10-01
卷期号:31 (10): 3361-8
被引量:10
摘要
While the benefit of passive immunotherapy is commonly accepted, active immunization may have advantages for the patient's quality of life. We identified a new epitope of Mab CH401 against Her-2/neu extracellular domain (N: 167-175), and evaluated the effect of active immunization of the 20mer peptide containing the epitope (CH401 peptide).Epitope-mapping was performed using ELISA with Her-2/neu-related multiple antigen peptides (MAP). BALB/c mice were transplanted with Her-2/neu-expressing lymphoma cell line and immunized with the peptides. For monitoring the condition, ELISA and flow cytometry was performed.CH401 peptide induced Her-2/neu-specific IgG antibody. Tumor growth in immunized mice was suppressed and tumor-infiltrating lymphocytes comprised more CD8(+) T-cells, which secreted larger amounts of interleukin-2 after the peptide re-stimulation.The new Her-2/neu peptide contained epitopes for CD4(+) and CD8(+) T-cells, which contributes to the suppressive effect on Her-2/neu-expressing tumor cell growth.
科研通智能强力驱动
Strongly Powered by AbleSci AI